SBIR Phase II: Indetification of Anaerobic Bacteria and their Metalbolic Capabilities for Bioremediation

小行星9500041 厌氧细菌具有生物降解一系列污染化学品的代谢活性，包括芳香族化合物、卤代芳香族化合物和卤代烯烃化合物。 厌氧菌是陆生细菌的重要组成部分，应被视为潜在的生物修复剂。然而，这些生物的知识，其多样性，分类和代谢能力，仍然处于初级水平。 本项目的目的是设计一种有效的，易于使用的，仪器化的测试试剂盒系统，可用于研究厌氧菌的分解代谢能力，并根据这些能力识别不同种类的厌氧菌。 为了开发这个试剂盒，将调查300多种厌氧菌的分解代谢潜力，并对至少300种不同的有机化合物进行编目，其中包括一些重要的异生物质化学物质。 从这些数据的计算机分析中，将选择一组95个碳源利用测试，以纳入商业微孔板测试试剂盒。 调查将提供一个概述的范围内的分解代谢活动的厌氧菌的问题。 如果成功的话，该检测试剂盒系统将在商业上销售给微生物学家，进行环境，工业和医学测试和厌氧菌鉴定。

9500041 Bochner Anaerobic bacteria possess metabolic activities for biodegrading a range of polluting chemicals including aromatic, halogenated aromatic, and halogenated alkene compounds. Anaerobes make up a significant portion of terrestrial bacteria and should be considered as potential agents of bioremediation. However, knowledge of these organisms, their diversity, taxonomy, and metabolic capabilities, is still at a rudimentary level. The purpose of this project is to design an efficient, easy-to-use, instrumented test kit system that can be used both to study the catabolic capabilities of anaerobes and to identify different species of anaerobes based on these capabilities. To develop this kit, the catabolic potential of over 300 species of anaerobes will be surveyed and cataloged against at least 300 different organic compounds, including some important xenobiotic chemicals. From computer analysis of these data, a set of 95 carbon source utilization tests will be selected to be incorporated into a commercial microplate test kit. The survey will provide an overview of the range of catabolic activities of the anaerobes in question. If successful, the test kit system will be marketed commercially to microbiologists doing environmental, industrial, and medical testing and identification of anaerobes.