Diversity and Metabolic Capabilities of Arctic Bacterioplankton Communities

小行星9500237 本项目将调查北冰洋浮游细菌群落组成与群落代谢能力之间的关系。 他们将研究北冰洋浮游细菌群落这些特性的空间变化模式，并试图将它们与促进异养生产的有机物质来源联系起来。 调查将使用1995年3月至5月从美国海军潜艇上收集的样本进行，该潜艇将专门用于北极海洋学。 巡航结束后，将在实验室对采集的样本进行分析。 将使用旧金山弗朗西斯科州立大学开发的基于分离16 S rRNA基因部分序列的方法分析群落组成。 社区代谢能力将通过细菌在95种不同有机碳源上生长的能力来评估。 他们将对水样进行化学分析，以确定氨基酸、碳水化合物和溶解有机碳的浓度，并利用天然细菌进行生物测定，以直接确定它们可以代谢的溶解有机碳的比例。

9500237 Hollibaugh This project will investigate the relationship between bacterioplankton community composition and community metabolic capabilities in the Arctic Ocean. They will examine the patterns of spatial variation in these properties of Arctic Ocean bacterioplankton communities and attempt to relate them to sources of organic matter fueling heterotrophic production. The investigation will be conducted using samples collected from a U.S. Navy submarine in March-May 1995, which will be dedicated to Arctic oceanography. Collected samples will be analyzed in the laboratory after the cruise. Community composition will be analyzed using a method developed at San Francisco State University based on separating partial sequences of the 16S rRNA gene. Community metabolic capabilities will be assessed by the ability of bacteria to grow on 95 different organic carbon sources. They will analyze water samples chemically to determine the concentrations of amino acids, carbohydrates and dissolved organic carbon, and conduct bioassays using native bacteria to determine directly the proportion of dissolved organic carbon that they can metabolize.