Modifiers of Dynein Function in Drosophila

这项拟议的研究是关于动力蛋白运动复合体如何在胚胎和体细胞中发挥作用的研究。研究人员将分离和鉴定显性“粘合”基因的基因外增强子和抑制子，并将从果蝇胚胎中纯化mRNP-动力蛋白-微管复合体。将进行基因敲除实验，以测试细胞质动力蛋白的作用。这项工作将与明尼苏达大学的托马斯·海斯博士合作进行。分子马达用于移动细胞内的组件，就像船上的舷外马达在水中移动船一样。申请者将在果蝇中使用可能的遗传方法来了解马达是如何工作的。***

Abstract K. Supernant The proposed research is an investigation of how the dynein motor complex functions in embryonic and somatic cells. The investigator will isolated and characterize extragenic enhancers and suppressors of the dominant "glued" gene and will purify mRNP-dynein-microtubule complexes from Drosophila embryos. Genetic knockout experiments will be conducted to test the role of cytoplasmic dynein. This work will be performed in association with Dr. Thomas Hays at the University of Minnesota. %%% Molecular motors serve to move components within cells much like a outboard motor on a boat moves the boat through the water. The applicant will employ genetic approaches possible in the fruit fly Drosophila to understand how the motor works. ***