G-Proteins in the Response System Phycomyces blakesleeanus

摘要9514026科恩是低等真菌Phycomyces blakesleanus的单细胞孢子囊团，对蓝光非常敏感，向光弯曲，在光照下生长，对光的减少表现出负生长反应。藻类真菌在比较低等生物体和高等生物体中的信号处理方面具有巨大的潜力。这位研究人员发现了G蛋白存在于孢子囊中的证据，G蛋白在高等生物的光转导和许多类型的信号转导中是必不可少的。在这项建议中，研究人员将试图确定G蛋白在藻类中蓝光转导中的作用。用G(亚基)和共价FAD(参与这种真菌的感光系统)的抗体进行免疫印迹，将用于一些行为突变株。将使用一个cDNA库来克隆G(亚基)，并将这些亚基与其他系统中的这些蛋白质进行比较，来自突变体的mRNAs将通过Northern blotting进行检测。这些研究将提供有关蓝光检测系统如何在分子水平上工作的重要信息，并将指明存在于低等真核生物和脊椎动物的信号转导系统之间的共同元件。***

Abstract 9514026 Cohen The single-cell sporangiophore of the lower fungus Phycomyces blakesleeanus is very sensitive to blue light, by bending toward the light and growing with exposure to light, and showing a negative growth response to a decrease in light. Phycomyces has great potential for comparing signal processing in lower organisms to those in higher organisms. This investigator has found evidence for the presence of G proteins in the sporangiophore, and G proteins are essential for phototransduction and many types of signal transduction in higher organisms. In this proposal the investigator will seek to establish the role of G proteins in blue light transduction in Phycomyces. Immunoblotting with antibodies to G( subunits and covalent FAD (which is involved in the photoreceptor system of this fungus), will be used in a number of behavioral mutant strains. A cDNA library will be used for cloning of G( subunits and these will be compared to these proteins in other systems, and mRNAs from the mutants will be examined by Northern blotting. These studies will provide important information on how the blue light detection system works at the molecular level and will indicate common elements that exists between signal transduction systems of the lower eukaryotic organisms and vertebrates. ***