Endocrine Regulation of the Ecdysteroid Cascade During Insect Metamorphosis

小行星9514187 蜕皮激素引起昆虫蜕皮和变态，保幼激素存在于幼虫生命期间以允许生长和蜕皮并防止变态。干扰这些激素的作用是控制害虫的一种方法。Riddiford博士发现，在烟草天蛾（Manduca sexta）的蜕皮和变态过程中，蜕皮类固醇受体亚型及其伴侣USP的变化存在一种动态而复杂的模式。 她现在计划研究这些受体的激素和时空调节以及它们在细胞水平上诱导的初始转录因子，主要使用免疫细胞化学和原位杂交分析体内和体外暴露于这些激素的组织。这些研究将提供更深入的了解蜕皮类固醇如何启动和协调细胞反应，导致在蜕皮过程中形成新的角质层，并在细胞命运的转换，这是必不可少的变态。通过研究保幼激素对这些细胞动力学的影响，她将揭示其在防止细胞命运转换中的作用模式。 ***

9514187 Riddiford Ecdysteroids cause insect molting and metamorphosis, and juvenile hormone is present during larval life to allow growth and molting and to prevent metamorphosis. Interference with the action of these hormones is one way to control insect pests. Dr. Riddiford has found that there is a dynamic and complex pattern of changing ecdysteroid receptor isoforms and its partner USP during a molt and at metamorphosis in the epidermis of the tobacco hornworm, Manduca sexta. She now plans to study both the hormonal and the spatiotemporal regulation of these receptors and the initial transcription factors that they induce at the cellular level using primarily immunocytochemical and in situ hybridization analysis of tissue exposed to these hormones both in vivo and in vitro. These studies will provide deeper insight into how the ecdysteroids initiate and coordinate the cellular responses that lead to the formation of a new cuticle during a molt and during the switch in cell fate that is essential for metamorphosis. By studying the influence of juvenile hormone on these cellular dynamics, she will shed new light on its mode of action in the prevention of the switch of cell fates. ***