Evaluation of DNA Probe-based Assays for Rapid Detection of Toxigenic Marine Diatoms

9602576学者蒙特利湾水族馆研究所的克里斯托弗·A·肖林和新西兰纳尔逊考松研究所的莱斯利·L·罗兹的这项合作研究项目的目标是：(1)确定结合大亚单位核糖体RNA(LSU RRNA)靶向探针的试纸条试验是否是鉴定和量化与软骨藻酸(DA)的产生有关的拟硝化细菌毒素种类的可行方法，软骨藻酸是一种与人类和野生动物的疾病(遗忘性贝类中毒)和经济损失有关的神经毒性物质，(2)比较试纸条试验和基于显微镜的全细胞荧光分析的性能，以及(3)评估作为新西兰贝类生物毒素监测项目一部分采集的样品的常规分析方法的实用性。这一结果将为人们如何使用DNA探针近乎实时地检测其他水生有毒微生物提供一个模型。

9602576 Scholin This objectives of this collaborative research project between Christopher A. Scholin of the Monterey Bay Aquarium Research Institute and Lesley L. Rhodes of the Cawthorn Institute in Nelson, New Zealand are (1) to determine whether the dipstick assay incorporating large-subunit ribosomal RNA (LSU rRNA)- targeted probes is a viable method for identifying and quantifying toxigenic species of Pseudo-nitzschia associated with the production of domoic acid (DA), a neurotoxic substance linked to illness of humans and wildlife (amnesic shellfish poisoning) and to economic losses, (2) to compare the performance of the dipstick assay to a microscopy-based whole cell fluorescence assay, and (3) to assess the utility of the method for routine analyses of samples collected as part of the New Zealand shellfish biotoxin monitoring program. The results will serve as a model for how one might detect other aquatic-borne toxic micro organisms in near real-time using DNA probes.