Controlling Sesquiterpene Synthase Gene Expression in Potato

9603168 Zook Zook博士是植物次生产物代谢生物化学方面的专家，特别关注倍半萜代谢。 该途径作为植物防御分子的主要生产者的重要性，推动了对该途径中的酶的生物化学理解到植物/病原体相互作用生物化学的最前沿。 该建议是关于一个精心策划的反义策略，其中定义的植物抗毒素，倍半萜合成的产物，或整个途径，通过反义技术消除。 此外，合成将通过过表达而增强。 模式生物是马铃薯，对植物的分析将包括在基因表达、生物化学和生理水平上的测量。 可以预期的是，这项工作产生的重要性或绝对必要性的phyoalexins的基础知识，并将提供信息的根本重要性，为我们了解植物/病原体的相互作用，以及如何在未来操纵这种相互作用。

9603168 Zook Dr. Zook is an expert in the biochemistry of plant secondary product metabolism with special focus on sesquiterpene metabolism. The importance of this pathway as the main producer of plant defense molecules has propelled the biochemical understanding of the enzymes in the pathway to the forefront of plant/pathogen interaction biochemistry. The proposal is about a well-planned antisense strategy with which defined phytoalexins, products of sesquiterpene synthesis, or the entire pathway, are eliminated by antisense technology. Additionally, synthesis will be enhanced by over-expression. The model organism is potato and the analysis of plants will include measurements at the gene expression, biochemical and physiological levels. It can be expected that the work produces fundamental knowledge about the importance or absolute essentiality of phyoalexins and will provide information of fundamental importance for our understanding of plant/pathogen interaction and how to manipulate such interaction in the future.