Dedicated Stopped-Flow Spectrometer for CD, Absorbance and Fluorescence Measurements

要求提供资金以购置一台停流分光计，用于吸光度、圆二色性和荧光测量。 项目将由来自生理学、生物物理学和化学系的六个主要用户进行。该仪器将用于研究蛋白质折叠，酶机制，肽和蛋白质与膜的相互作用。McLaughlin教授将研究蛋白质Src和MARCKS与膜的相互作用。米勒教授将研究多结构域蛋白质的折叠和组装。 罗利教授将使用这种仪器来帮助阐明两种蛋白质的折叠途径。桑普森教授将研究胆固醇氧化酶的重要酶的机制。 Scarlata教授将研究G蛋白对磷脂酶C的激活作用。Wishina教授将研究蛋白质熔融球态的动力学。 仪器将放置在化学系的共享仪器实验室中，并作为用户可访问、操作员维护的共享设施运行。

Funds are requested for the acquisition of a stopped-flow spectrometer for absorbance, circular dichroism and fluorescence measurements. Projects will be conducted by six major users from the Departments of Physiology & Biophysics and Chemistry. The instrument will be used in investigations of protein folding, enzyme mechanisms, peptide and protein interactions with membranes. Prof. McLaughlin will study the interactions of the proteins Src and MARCKS with membranes. Prof. Miller will study the folding and assembly of multidomain proteins. Prof. Raleigh will use this instrumentation to help elucidate the folding pathway of two proteins. Prof. Sampson will study the mechanism of the important enzyme cholesterol oxidase. Prof. Scarlata will investigate the activation of phospholipase C by G proteins. Prof. Wishina will study the dynamics of molten globule states of proteins. The instrument will be housed in a shared instrumentation laboratory located in the Chemistry Department and operated as a user-accessible, operator maintained shared facility.