Replacement of a 500 MHz NMR Console at Florida State University

申请资金用于替换现有的瓦里安VXR 500- s 500 MHz核磁共振光谱仪控制台，该控制台是佛罗里达州立大学化学系核磁共振设施的一部分。我们的目标是将现有的控制台替换为具有三个独立射频（rf）通道，每个rf通道的波形发生器和三轴脉冲场梯度附件的控制台。为了利用额外的硬件功能，我们还将购买总共三个新的双共振和三共振探头和一个离线硅图形印第工作站，配备一个外部硬盘驱动器和一个CD录音机。具有非常好的漂移和线形规格的11.7 T牛津超导磁体不会被替换。为了优化生物样品中水共振的线形，我们将购买一套新的室温垫片。这一行动方针将以具有成本效益的方式大大提高该工具的全面能力，同时保留现有系统的适当功能组成部分。替换的光谱仪控制台将用于(1)糖蛋白结构生物学，(2)蛋白质折叠，(3)蛋白质-溶剂相互作用，(4)RNA结构，(5)RNA -蛋白质复合物的结构，(6)较大的有机分子的溶液研究。

Funds are requested to replace an existing Varian VXR 500-S 500 MHz NMR spectrometer console, which is part of the Chemistry Department NMR Facility at Florida State University. Our objective is to replace the existing console with one having three independent radio frequency (rf) channels, waveform generators for each rf channel, and a three-axis pulsed-field gradient accessory. To take advantage of the additional hardware capabilities, we will also purchase a total of three new double- and triple-resonance probes and an off-line Silicon Graphics Indy workstation out-fitted with an external hard-drive and a CD recorder. The 11.7 T Oxford super-conducting magnet, which has very good drift and lineshape specifications, will not be replaced. To optimize the lineshape of the water resonance in biological samples, a new room-temperature shim set will be purchased. This course of action will substantially enhance the overall capabilities of the instrument in a cost-effective manner, while retaining the desirable functional components of the existing system. The replacement spectrometer console will be used for solution studies of ( 1 ) glycoprotein structural biology, (2) protein folding, (3) protein-solvent interactions, (4) RNA structure, (5) structures of RNAprotein complexes, and (6) larger organic molecules.