An Automated DNA Sequencer for Molecular Phylogeny and Systematics, Population Genetics, Molecular Evolution, and Development

请求提供支助，以购买ABI Prism 377自动DNA测序仪，作为范德比尔特大学生物系五名分子和进化生物学家的主要研究工具。 我们的研究小组目前严重依赖手动测序，但最近的研究需求和新的研究进展需要使用高通量自动测序。ABI Prism 377能够持续、准确地提供高达我们现在所能实现的10倍的数据吞吐量，这将代表我们实验室研究能力的巨大提高。 五位PI/主要用户将在ABI 377自动测序仪上进行一系列项目，探索生物体和分子进化中的各种问题：1）光合和非光合植物细胞器DNA进化的系统发育和机制研究（dePamph 1 lis），2）物种相互作用和共同进化的分子系统发育研究（Pellmyr），3）植物和蓝藻生物钟基因的进化（约翰逊），4）细胞器DNA多态性和集合种群的遗传学（麦考利），5）脊索动物发育的进化（斯瓦拉）。

Support is requested to purchase an ABI Prism 377 automated DNA sequencer to be used as a primary research tool for five molecular and evolutionary biologists in the Biology Department at Vanderbilt University. Our research group currently relies heavily on manual sequencing, but recent research needs and new research developments require the use of high-throughput automated sequencing. The ABI Prism 377, which is capable of sustained, accurate data throughput of up to 10 times what we can now accomplish, would represent a quantum improvement in research capability for our laboratories. The five PIs / Major Users will conduct a series of projects on the ABI377 autosequencer that explore diverse problems in organismal and molecular evolution: 1) Phylogenetic and mechanistic studies of organelle DNA evolution in photosynthetic and nonphotosynthetic plants (dePamph1lis), 2) Molecular phylogenetic studies of species interactions and coevolution (Pellmyr), 3) Evolution of genes underlying circadian clocks in plants and cyanobacteria (Johnson), 4) Organelle DNA polymorphism and the genetics of metapopulations (McCauley), and 5) Evolution of development in chordates (Swalla).