Collaborative Research: The Green Plant BAC Library Project

合作研究：绿色植物BAC图书馆项目

• 批准号: 0207202
• 负责人: Claude dePamphilis
• 金额: $ 7.18万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0207202&HistoricalAwards=false
• 关键词: Collaborative; Research; Green; Plant; BAC

0207202de PamphillisThis research project is designed to accomplish two aims: Aim 1: To construct and array high-quality BAC libraries to provide a genomic resource on a wide range of species. Aim 2: To enable researchers working with green algae, non-seed land plants, and seed plants (including flowering plants) to identify genes critical for understanding plant form and function and how land plants arose and diversified. This project will enable progress toward an understanding of the genetic basis for the transitions that mark the most fundamentally important steps in green plant evolution. The Deep Green community (http://ucjeps.herb.berkeley.edu/bryolab/deepgene/index.html) will help to provide an infrastructure for ongoing scientific exchange. Bioinformatics and a web site will be provided to the community to access these resources (http://www.genome.clemson.edu/). Selected Species: Desired coverage is given in parentheses. Where two coverage values are listed, two libraries using different restriction enzymes will be made.Green algae: Volvox carteri (5x, 5x) Caulerpa mexicana (8x)Mesostigma viride (8x)Coleochaete orbicularus (6.4x, 6.4x)Chara aspera (6x)Non-seed plants: Marchantia polymorpha (8x)Anthoceros sp. (6.3x, 6.3x)Lycopodium lucidulum (5.1x, 5.1x)Angiopteris erecta (6.2x, 6.2x)Ceratopteris richardii (4.8x, 4.8x)Marsilea quadrifolia (5.6x, 5.6x) Seed plants:Amborella trichopoda (5.5x)Nuphar adventa (7x)Acorus gramineus (7x)Lirodendron tulipifera (7x)Mimulus guttatus (7x)

0207202 de Pamphillis本研究项目旨在实现两个目标：目标1：构建和排列高质量的BAC文库，为广泛的物种提供基因组资源。 目标二：使研究人员能够与绿色藻类，非种子陆地植物和种子植物（包括开花植物）合作，以确定对理解植物形态和功能以及陆地植物如何出现和多样化至关重要的基因。 这个项目将使进展走向理解的遗传基础的转变，标志着最根本的重要步骤，在绿色植物进化。 深绿色社区（http：//ucjeps.herb.berkeley.edu/bryolab/deepgene/index.html）将帮助为正在进行的科学交流提供基础设施。 将向社区提供生物信息学和一个网站，以获取这些资源（http：//www.genome.clemson.edu/）。 选定物种：括号内为所需覆盖范围。 在列出两个覆盖度值的情况下，将使用不同的限制酶制备两个文库。绿色藻类：团藻（Volvox carteri）（5x，5x）墨西哥蕨藻（Caulerpa mexicana）（8x）绿色中柱藻（Mesostigma viride）（8x）圆形鞘毛藻（Coleochaete orbicularus（6.4x，6.4x）Chara aspera（6x）非种子植物：多形地钱（8x）Anthoceros sp.（6.3x，6.3x）Lycopodium lucidulum（5.1x，5.1x）观音座莲（6.2x，6.2x）Ceratopteris richardii（4.8x，4.8x）Marsilea quadrifolia（5.6x，5.6x）种子植物：Amborella sputpoda（5.5x）Nuphar adventa（7x）Acorus gramineus（7x）Lirodendron tuthefera（7x）Mimulus guttatus（7x）