U.S.-Swedish Cooperative Research: Redox Titration of Protein Phosphorylation in Photosynthetic Membranes

PI和隆德大学的约翰艾伦之间的国际合作， 瑞典有两个目标。 一个是进一步表征氧化还原控制的 来自高等植物、蓝细菌叶绿体的膜蛋白的磷酸化 类囊体膜和紫色细菌的色素细胞。 的主要方法 将使用电位氧化还原滴定和磷光成像。 二是 确定单个磷蛋白在控制光能收集中的作用 以及光合电子传递。 那里使用的主要方法是 将是荧光光谱探测单周转和稳态电子 转移 隆德实验室将提供执行所需的设备和资源 研究 合作的结果可能会导致我们的突破 了解光合作用的基本过程，并可应用于 其他细胞系统。

This international collaboration between the PI and John Allen of Lund University, Sweden has two objectives. One is to further characterize redox control of the phosphorylation of membrane proteins from chloroplasts of higher plants, cyanobacterial thylakoid membranes, and chromatophores from purple bacteria. The primary method used will be potentiometric redox titration and phosphorimaging. The other is to determine the roles of individual phosphoproteins in controlling light-energy harvesting and distribution, as well as photosynthetic electron transfer. The main method used there will be fluorescence spectroscopy to probe both single turnover and steady-state electron transfer. The Lund laboratory will provide equipment and resources needed to perform the research. The result of the collaboration could lead to a breakthrough in our understanding of a process fundamental to photosynthesis, and could have application to other cell systems.