Purchase of Instrumentation for Peptide Synthesis and Purification

这份来自化学研究仪器和设施项目的合同将帮助位于博尔德的科罗拉多大学化学系获得一台自动化固相肽合成器和一台高效液相色谱仪。该设备将加强许多领域的研究，包括：(1)利用肽探测蛋白质信号的分子间调节的研究，(2)糖基化对肽构象的结构影响的研究，(3)磷酸肽类似物的合成和表征，(4)蛋白质折叠中间体和蛋白质设计的结构表征，以及(5)MAP激酶信号转导途径的研究。肽合成器和高效液相色谱仪（HPLC）被生物科学家用来制备和纯化各种蛋白质的天然底物/效应器的类似物。

This award from the Chemistry Research Instrumentation and Facilities Program will assist the Department of Chemistry at the University of Colorado at Boulder to acquire an automated solid- phase peptide synthesizer and a high performance liquid chromatograph (HPLC). This equipment will enhance research in a number of areas including the following: (1) studies utilizing peptides to probe intermolecular tuning of protein signaling, (2) studies of the structural ramifications of glycosylation on peptide conformation, (3) synthesis and characterization of phosphopeptide analogues, (4) structural characterization of the protein folding intermediates and protein design, and (5) studies of the MAP kinase signal transduction pathway. The peptide synthesizer and the high performance liquid chromatograph (HPLC) are used by biological scientists to make and purify analogs of the natural substrates/effectors of a variety of proteins.