Role of Pto- and Fen-interacting Proteins in Plant Disease Resistance and Fenthion Sensitivity

摘要9630635马丁，这项研究的目的是详细了解参与植物抗病的病原菌识别和信号转导事件。番茄上的两个实验系统正在研究中，它们是对紫丁香假单胞菌的抗性。番茄(细菌性斑点病的病原体)由PTO基因赋予，对杀灭硫磷杀虫剂的敏感性由Fen基因赋予。PTO和Fen基因是编码密切相关的丝氨酸/苏氨酸蛋白激酶的一个小基因家族的成员。在这项拟议的研究中，将对几个已被证明编码与PTO激酶物理上相互作用的蛋白质的基因进行表征。这些蛋白质将使用生化技术和转基因方法进行检测。此外，还将识别受PTO和Fen激酶调控的基因，并将用它们来研究植物抵御病原体的途径。这些研究将有助于我们理解识别和信号转导事件的分子基础，这些事件涉及植物对病原体的防御。***

Abstract 9630635 Martin The goal of this research is to gain a detailed understanding of pathogen recognition and signal transduction events involved in plant disease resistance. Two experimental systems in tomato are being studied, resistance to Pseudomonas syringae pv. tomato (causative agent of bacterial speck disease) conferred by the Pto gene and sensitivity to the fenthion insecticide conferred by the Fen gene. The Pto and Fen genes are members of a small gene family encoding closely related serine/threonine protein kinases. In the proposed research several genes that have been shown to encode proteins that physically interact with the Pto kinase will be characterized. These proteins will be examined using biochemical techniques and transgenic approaches. In addition, genes will be identified which are regulated by the Pto and Fen kinases, and they will used to examine the pathways whereby plants defend themselves from pathogens. These studies will aid in our understanding of the molecular basis of recognition and signal transduction events involved in plant defense against pathogens. ***