Collaborative Research: Recombinant Production of Enzymes from Hyperthermophiles: Effects of High Temperature and Pressure

9632554 Robb这项建议是为了研究从嗜热微生物中开发酶和其他蛋白质的两个关键问题，这两个问题是：(1)开发加工方法，以获得大量这些酶，以供进一步研究和潜在应用，以及(2)研究压力诱导极端嗜热菌蛋白质稳定的机制，并设计出操纵这些酶的热稳定性的方法。定点突变是一种系统化的方法，与高压下的生物催化研究一起用来阐明压力对蛋白质热稳定性的影响以及这些多聚体酶中亚基的相互作用。***

9632554 Robb This proposal is on research to examine two key issues in the development of enzymes and other proteins from thermophilic microorganisms which are to: (1) develop processing methods to obtain significant quantities of these enzymes for further investigation and potential application, and (2) examine the mechanisms of pressure-induced stabilization of proteins from extreme thermophiles and devise approaches to manipulate the thermostability of these enzymes. Site-directed mutagenesis is to be used in a systematic way, along with biocatalytic studies at elevated pressures to elucidate the effects of pressure on protein thermostability and the interaction of subunits in these multimeric enzymes. ***