Fundamental Separations Studies of Pressure-Induced Perturbations in Complexation

克里斯汀·埃文斯在分析和表面化学计划的支持下，研究了压力作为变量对β-环糊精络合物结合常数的影响，并确定了压力对此类络合物的色谱保留和选择性的影响。这种主-客体相互作用形成了困难的位置异构体和对映体物种的液相色谱和电泳法分离的基础。将使用液相色谱、核磁共振和荧光测量来检查压力效应。将探索高压毛细管电泳法，并将其用于测量这些络合反应的压力依赖性。这项工作探索了在含有β-环糊精的络合物的化学和分离中使用压力作为变量。更好地了解这些络合物的溶液化学和改进它们的分离方法，可能会对制备纯药物和其他化学纯度和结构相同的化合物产生积极的影响。

Christine E. Evans is supported by the Analytical and Surface Chemistry Program to study the effects of pressure as a variable on the binding constants of beta-cyclodextrin complexes and to determine the effects of pressure on chromatographic retention and selectivity of such complexes. Such host-guest interactions form the basis for liquid chromatographic and electrophoretic separations of difficult positional isomers and enantiometric species. Liquid chromatographic, nuclear magnetic resonance, and fluorescence measurements will be used to examine the pressure effects. Capillary electrophoresis at high pressure will be explored and also used in the measurement of the pressure dependence of these complexation reactions. This work explores the use of pressure as a variable in the chemistry and separation of complexes involving beta-cyclodextrin. A better understanding of the solution chemistry of these complexes and improved methods for their separation has the potential to impact positively on the preparation of pure pharmaceuticals and other compounds which are needed in a high state of chemical purity and structure sameness.