Macroporous Chitin and Chitosan Membranes for Affinity Separation of Biomolecules

摘要 提案编号： 9711389 提案类型： 研究者发起的主要研究者： Eli Ruckenstein Affiliation： 布法罗纽约州立大学 该补助金通过化学和运输系统司界面，运输和分离计划的分离和纯化计划子要素授予。主要研究者是布法罗纽约州立大学的Eli Ruckenstein博士。本研究的目的是利用甲壳素和壳聚糖这两种生物相容性材料来开发具有可控孔隙率的亲和膜。产生孔隙率所遵循的方法利用了这样一个事实：聚合物和致孔剂具有作为溶液pH值的函数的广泛不同的溶解度特征。溶解度差异用于首先在酸性pH值下浇铸膜，然后在碱性pH值下去除致孔剂。 在形成膜之后，研究的目的是表征膜的应用潜力，特别是亲和分离。 该研究可能导致制备廉价的、生物相容的甲壳素/壳聚糖膜，其在体内治疗、组织培养、伤口愈合和亲和分离中具有多种应用。

Abstract Proposal No: 9711389 Proposal Type: Investigator Inititated Principal Investigator: Eli Ruckenstein Affiliation: State University of New York at Buffalo This grant is awarded through the Separations and Purification Program sub-element of the Interfacial, Transport and Separations Program of the Chemical and Transport Systems Division. The principal investigator is Dr. Eli Ruckenstein of the State University of New York at Buffalo. The objective of the research is to develop affinity membranes of controlled porosity using chitin and chitosan, which are biocompatible materials. The approach followed to generate porosity takes advantage of the fact that the polymer and a porogen have widely different solubility characteristics as a function of solution pH. The solubility difference is used to first cast the membrane at acid pH values and then remove the porogen at basic pH values. After forming the membrane, the objectives of the research are to characterize the application potential of the membranes, particularly to affinity separations. The research could lead to the preparation of inexpensive, biocompatible chitin/chitosan membranes have a variety of applications for in vivo therapies, tissue culture, wound healing, and affinity separations.