Macroporous Cellulose Membrane for Affinity Chromatography

摘要以滤纸为原料，采用交联法制备了大孔纤维素膜。利用这些高多孔膜，进一步进行化学修饰，以获得含有特定蛋白质或酶吸附选择性配体的亲和膜。最终目标是生产亲和盒，可用于高选择性地分离许多蛋白质。随着治疗性蛋白质在医学上的广泛应用，需要高效、有效的方法来经济地生产这些蛋白质。大孔亲和膜通过在更低的压降下提供更高的速率提供了优于传统色谱的优点。交联纤维素膜具有机械耐用性和化学稳定性以及生物相容性。它们可以用在墨盒中进行选择性蛋白质分离和纯化，并通过引入特定的配体进行定制。

ABSTRACTMacroporous cellulose membranes are being prepared from filter paper by cross-linking. With these highly porous membranes, further chemical modifications are carried out in order to obtain affinity membranes containing selective ligands for specific protein or enzyme adsorption. The final goal is to produce affinity cartridges which can be used to separate with high selectivity a number of proteins. As therapeutic proteins find wider use in medicine, efficient and effective methods are required to produce these proteins economically. Macroporous affinity membranes offer advantages over conventional chromatography by providing higher rates at lower pressure drops. The cross-linked cellulose membranes are mechanically durable and chemically stable as well as biocompatible. They can be used in cartridges for selective protein separation and purification and tailored by introduction of specific ligands.