Macroporous Cellulose Membrane for Affinity Chromatography
Macroporous Cellulose Membrane for Affinity Chromatography
批准号:
0086687
负责人:
Eli Ruckenstein
金额:
$25.0万
依托单位:
依托单位国家:
美国
项目类别:
Continuing Grant
财政年份:
2001
资助国家:
美国
项目状态:
已结题
起止时间:
2001-01-01 至 2003-12-31
中文摘要
摘要以滤纸为原料,采用交联法制备了大孔纤维素膜。利用这些高多孔膜,进一步进行化学修饰,以获得含有特定蛋白质或酶吸附选择性配体的亲和膜。最终目标是生产亲和盒,可用于高选择性地分离许多蛋白质。随着治疗性蛋白质在医学上的广泛应用,需要高效、有效的方法来经济地生产这些蛋白质。大孔亲和膜通过在更低的压降下提供更高的速率提供了优于传统色谱的优点。交联纤维素膜具有机械耐用性和化学稳定性以及生物相容性。它们可以用在墨盒中进行选择性蛋白质分离和纯化,并通过引入特定的配体进行定制。
英文摘要
ABSTRACTMacroporous cellulose membranes are being prepared from filter paper by cross-linking. With these highly porous membranes, further chemical modifications are carried out in order to obtain affinity membranes containing selective ligands for specific protein or enzyme adsorption. The final goal is to produce affinity cartridges which can be used to separate with high selectivity a number of proteins. As therapeutic proteins find wider use in medicine, efficient and effective methods are required to produce these proteins economically. Macroporous affinity membranes offer advantages over conventional chromatography by providing higher rates at lower pressure drops. The cross-linked cellulose membranes are mechanically durable and chemically stable as well as biocompatible. They can be used in cartridges for selective protein separation and purification and tailored by introduction of specific ligands.
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Thermally Reversible Covalent Linking of Halide-containing Polymers
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批准号:0086491
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项目类别:Standard Grant
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资助金额:$16.71万
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财政年份:2000
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负责人:Eli Ruckenstein
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依托单位:
Collaborative Research: Statistical Study of the Homogeneous Nucleation by Applying a Novel Multidroplet Levitator Trap and Microscopic Theory of Homogeneous Nucleation
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批准号:0000548
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项目类别:Standard Grant
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资助金额:$39.88万
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财政年份:2000
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负责人:Eli Ruckenstein
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依托单位:
Amphiphilic Particles
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批准号:9712982
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项目类别:Standard Grant
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资助金额:$5.5万
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财政年份:1998
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负责人:Eli Ruckenstein
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依托单位:
Macroporous Chitin and Chitosan Membranes for Affinity Separation of Biomolecules
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批准号:9711389
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项目类别:Standard Grant
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资助金额:$20.0万
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财政年份:1997
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负责人:Eli Ruckenstein
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依托单位:
GOALI: "Compatibilized Polymer Blends"
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批准号:9616192
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项目类别:Continuing Grant
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资助金额:$21.91万
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财政年份:1997
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负责人:Eli Ruckenstein
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依托单位:
Dynamics and "Phase Behavior" of Foams and Concentrated Emulsions
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批准号:9522710
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项目类别:Continuing Grant
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资助金额:$18.0万
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财政年份:1996
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负责人:Eli Ruckenstein
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依托单位:
Chitosan-Based Affinity Separation Membranes
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批准号:9629458
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项目类别:Standard Grant
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资助金额:$3.5万
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财政年份:1996
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负责人:Eli Ruckenstein
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依托单位:
A New Technology, Based on Inversted Micelles, for Composite Conductive Polymers
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批准号:9406889
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项目类别:Standard Grant
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资助金额:$3.5万
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财政年份:1994
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负责人:Eli Ruckenstein
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依托单位:
Microsponge Molecular Reserviors and Their Use in the Heterogenization of Homogeneous Catalysts
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批准号:9301711
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项目类别:Continuing Grant
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资助金额:$21.0万
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财政年份:1993
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负责人:Eli Ruckenstein
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依托单位:
Modification of the Surface Properties of Polymers
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批准号:9203384
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项目类别:Standard Grant
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资助金额:$9.27万
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财政年份:1992
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负责人:Eli Ruckenstein
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依托单位:
Emulsion Pathways to Composite Polymeric Membranes for Separation Processes
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批准号:8909948
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项目类别:Continuing Grant
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资助金额:$30.63万
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财政年份:1989
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负责人:Eli Ruckenstein
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依托单位:
Role of Transport Phenomena in the Impregnation of Porous Media by Active Catalytic Materials
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批准号:8806288
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项目类别:Standard Grant
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资助金额:$10.5万
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财政年份:1988
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负责人:Eli Ruckenstein
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依托单位:
A New Kind of Membrane for Separation Processes: The Hydrophobic-Hydrophilic Membranes (Expedited Award for Novel Research)
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批准号:8900259
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项目类别:Standard Grant
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资助金额:$3.0万
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财政年份:1988
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负责人:Eli Ruckenstein
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依托单位:
Fouling Employed to Design Adsorbents
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批准号:8716650
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项目类别:Standard Grant
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资助金额:$3.95万
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财政年份:1987
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负责人:Eli Ruckenstein
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依托单位:
A Surface Energetic Approach to Minimize the Biofouling of Solid Surfaces
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批准号:8415315
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项目类别:Continuing Grant
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资助金额:$16.21万
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财政年份:1985
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负责人:Eli Ruckenstein
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依托单位:
Thermodynamics of Solubilization in Aqueous Surfactant Solutions and Separation Processes Based on Solubilization
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批准号:8219639
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项目类别:Continuing Grant
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资助金额:$20.06万
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财政年份:1983
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负责人:Eli Ruckenstein
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依托单位:
Potential Barrier Chromatography - High Performance Liquid Chromatography Method For Protein Separations
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批准号:8117734
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项目类别:Continuing Grant
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资助金额:$35.28万
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财政年份:1982
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负责人:Eli Ruckenstein
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依托单位:
Surfactant Aggregation in Aqueous and Non-Aqueous Media
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批准号:7920308
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项目类别:Standard Grant
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资助金额:$6.5万
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财政年份:1980
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负责人:Eli Ruckenstein
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依托单位:
Solubilization and Microemulsions
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批准号:7920831
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项目类别:Standard Grant
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资助金额:$12.42万
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财政年份:1980
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负责人:Eli Ruckenstein
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依托单位:
Separation Methods of Colloidal Particles Based on Adsorption-Desorption
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批准号:7807129
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项目类别:Standard Grant
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资助金额:$7.5万
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财政年份:1978
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负责人:Eli Ruckenstein
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依托单位:
国内基金
海外基金
LBL改性PCL-Cellulose纳米支架激活Kc细胞的Integrin-FAK信号通路机制研究
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批准号:81401597
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项目类别:青年科学基金项目
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资助金额:23.0万元
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批准年份:2014
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负责人:黄容
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依托单位: