Macroporous Cellulose Membrane for Affinity Chromatography

用于亲和色谱的大孔纤维素膜

• 批准号: 0086687
• 负责人: Eli Ruckenstein
• 金额: $ 25万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0086687&HistoricalAwards=false
• 关键词: Macroporous; Cellulose; Membrane; Affinity; Chromatography

ABSTRACTMacroporous cellulose membranes are being prepared from filter paper by cross-linking. With these highly porous membranes, further chemical modifications are carried out in order to obtain affinity membranes containing selective ligands for specific protein or enzyme adsorption. The final goal is to produce affinity cartridges which can be used to separate with high selectivity a number of proteins. As therapeutic proteins find wider use in medicine, efficient and effective methods are required to produce these proteins economically. Macroporous affinity membranes offer advantages over conventional chromatography by providing higher rates at lower pressure drops. The cross-linked cellulose membranes are mechanically durable and chemically stable as well as biocompatible. They can be used in cartridges for selective protein separation and purification and tailored by introduction of specific ligands.

摘要大孔纤维素膜是由滤纸通过交联制备的。 利用这些高度多孔的膜，进行进一步的化学改性以获得含有用于特定蛋白质或酶吸附的选择性配体的亲和膜。 最终目标是生产亲和柱，其可用于以高选择性分离许多蛋白质。随着治疗性蛋白质在医学中的广泛应用，需要高效和有效的方法来经济地生产这些蛋白质。 大孔亲和膜通过在较低压降下提供较高的速率而提供优于常规色谱的优点。 交联纤维素膜具有机械耐久性和化学稳定性以及生物相容性。 它们可用于选择性蛋白质分离和纯化的柱中，并通过引入特异性配体进行定制。