Thermophilic Enzyme Hydrolysis of Water-Soluble Polymers: Interrelationship Between Biocatalysis and Rheology

9711781以半乳甘露聚糖为基础的天然聚合物的Prud‘homme水溶液被广泛用作水力压裂液，以提高气井和油井的产能。半乳甘露聚糖聚合物的化学或酶解是清除聚合物残留物和打开裂缝以供石油或天然气流动所必需的。由于环境和工艺的原因，半纤维素酶的使用已成为首选的水解法。然而，人们对这些酶降解半乳甘露聚糖的机理以及这些体系的生物催化、物理化学和流变学之间的关系知之甚少。这项合作研究的目的是了解现有的和建议的聚合物压裂溶液在高温下酶解过程中的物理化学和流变学。结果是生产新一代刺激液，将增加可利用的石油和天然气储量，展示一种新类别或耐温和热活性酶的技术效用，并提供对半乳甘露聚糖溶液和凝胶的水解动力学和机理的基本了解。***

9711781 Prud'homme Aqueous solutions of galactomannan-based natural polymers are used extensively as hydraulic fracturing fluids to enhance productivity from gas and oil wells. Chemical or enzymatic hydrolysis of the galactomannan polymers is necessary to remove the polymer residue and open the fracture for oil or gas flow. For environmental and processing reasons, the use of hemicellulosic enzymes has become the preferred route for hydrolysis. However, little is known about the mechanisms by which these enzymes hydrolyze galactomannans or about the relationship among biocatalysis, physical chemistry, and rheology of these systems. The objectives of this collaborative study are to understand the physical chemistry and rheology of existing and proposed polymeric fracturing solutions during enzymatic hydrolysis at high temperatures. Results are to produce a new generation of stimulation fluids that would increase accessible oil and gas reserves, demonstrate the technological utility of a new class or termostable and thermoactive enzymes, and provide a fundamental understanding of the kinetics and mechanism of hydrolysis of galactomannan solutions and gels. ***