CAREER: Genetic, Molecular and Physiological Studies of Lateral Root Development in Arabidopsis thaliana

9722191威廉斯育种计划由于难以接近，通常忽略了对根系的改造。不可能对诸如根结构或结构等进行直接选择。选择方案是间接的。拟南芥是鉴定根构型调控基因的理想模型。为了鉴定侧根形成率异常的根，将筛选M2诱变种子。只有那些原生根的生长速度和形态没有改变的植物才会被研究。将对剩余的突变体进行广泛的遗传分析，包括回交到野生型，以及构建双突变体组合。选择的基因座将被克隆。此外，选择基因的保守区域将用于侧根cDNA文库的扩增。根特异性基因的表达模式和编码基因产物的功能将被确定。建议的工作将主要由本科生，在暑期课程和所需的高级研究项目中进行。这个项目将有助于更好地了解侧根形成的可塑性和参与侧根形成的基因。

9722191 Williams Breeding programs have typically neglected, due to its inaccessibility, modification of the root system. No direct selection is possible for the improvement of, e.g., root structure or architecture. Selection schemes are indirect. Arabidopsis is an ideal model in which to identify the genes controlling root architecture. To identify roots with abnormal rates of lateral root formation, M2 mutagenized seeds will be screened. Only those plants in which the growth rate and morphology of the primary root is not altered will be pursued. An extensive genetic analysis of the remaining mutants will be carried out, including backcrosses into the wild type, and construction of double mutant combinations. Selected loci will be cloned. In addition, conserved regions of select genes will be used for the amplification of a lateral root cDNA library. The expression pattern of the root-specific genes and the function of the encoded gene products will be ascertained. The proposed work will be conducted primarily by undergraduate students, in summer programs and in required senior research projects. A better understanding of the plasticity of lateral root formation and of genes involved in lateral root formation will result from this project.