Cloning and Characterization of a New Locus RGA Involved in Gibberellin Signal Transduction in Arabidopsis

97-23171太阳赤霉素是一个二萜类化合物大家族，其中许多是植物中的生长调节剂，影响植物的不同发育过程，如发芽、茎伸长、叶展开、花和果实或种子的发育。拟议的工作集中在细胞对赤霉素的感知和信号转导途径，该信号转导途径导致已知的激素反应，从而导致基因表达的变化，以及发育和生理反应的很好描述。相比之下，人们对赤霉素的感知和信号知之甚少。这项提议将描述基因RGA，它很可能编码赤霉素受体，并研究拟南芥中在赤霉素信号转导的不同方面存在缺陷的突变体。这项工作包括遗传分析、基因克隆和基因表征的传统技术、表达的细胞特异性，最后是用于基因功能描述的生化分析。虽然这项工作主要是为了获得对激素信号的理解，但例如，作物改良工作的应用方面是相当可观的。

97-23171 Sun Gibberellins are a large family of diterpenoid compounds, many of which are growth regulators in plants which affect diverse developmental processes, such as germination, stem elongation, leaf expansion, or flower and fruit or seed development. The proposed work focuses on the perception of gibberellins by cells and the signal transduction pathway that leads to the known responses of the hormone resulting in changes in gene expression and the developmental and physiological reactions that are well described. Little, in contrast, is known about gibberellin perception and signaling. The proposed will characterize the gene, RGA, which very likely encodes a gibberellin receptor, and study mutants in Arabidopsis which are defective in different aspects of gibberellin signal transduction. The work includes genetic analysis, gene cloning and the traditional techniques for characterizations of genes, cell-specificity of expression and, finally, biochemical analyses for a functional description of genes. While the work is principally designed for obtaining an understanding of hormone signaling, the application aspects of the work for crop improvement, for example, are considerable.