SGER: Efficient Extrachromosomal Replication of Exogenous DNA by a Filamentous Fungus

这项探索性研究小额资助是基于一项发现，即丝状真菌拟盘多毛孢具有将非复制的转化DNA转化为自我复制的质粒的独特能力。就效率和机制而言，这与基本上所有其他丝状真菌通过染色体整合复制转化DNA形成鲜明对比。小孢子假单胞菌的高效转化和自复制质粒的产生这两个方面，如果适用于其他丝状真菌，可能会对这类重要生物的分子基因工程领域产生重大影响。本研究发展了小孢子假单胞菌质粒的扩增方法，这些质粒的结构和功能特征，以及测试它们在其他丝状真菌中的复制能力的实验。

Long 9724999 This Small Grant for Exploratory Research is based on the discovery that the filamentous fungus, Pestalotiopsis microspora, has a unique ability to convert non-replicating transforming DNA into self-replicating plasmids. This is in stark contrast, in terms of both efficiency and mechanism, to essentially all other filamentous fungi which replicate transforming DNA through chromosomal integration. Two aspects of P. microspora transformation, high efficiency and generation of self-replicating plasmids, could, if applicable to other filamentous fungi, have substantial impact on the growing field of molecular genetic engineering of this important class of organisms. This research develops methods for amplification of P. microspora plasmids, structural and functional characterization of these plasmids, and experiments to test their ability to replicate in other filamentous fungi.