Insect Cold-Hardiness and Diapause: Regulatory Relationships

pi之间的合作研究将集中在昆虫越冬生存的生理机制上。本研究探讨了两种昆虫利用的机制，一种不能忍受冷冻（肉蝇），另一种可以忍受广泛的内部冷冻（瘿蝇幼虫）。该项目的第一部分将描述冷休克（应激）蛋白及其基因在耐寒性中的作用。第二部分考察了细胞冷硬化和细胞内抗冻性的性质。最后，pi将研究甘油在保护神经系统免受冷休克损伤中的作用。这项工作不仅将提供对耐寒性新机制的理解，而且可能为害虫种群的破坏以及昆虫和其他生物在低温下的长期储存提供新的工具。

Lee 9728573 Collaborative research between the PIs' will focus on physiological mechanisms used by insects for overwinter survival. This study examines mechanisms utilized by two types of insects, one that can not tolerate freezing (a flesh fly) and one that tolerates extensive internal freezing (the gall fly larvae). The first part of the project will characterize cold shock (stress) proteins and their genes in relation to their role in cold tolerance. The second part examines the nature of cellular cold hardening and intracellular freeze tolerance. Lastly the PIs' will investigate the role of glycerol in protecting the nervous system against cold shock injury. This work will not only provide an understanding of new mechanisms of cold tolerance, but may also provide new tools both for the disruption of insect pest populations, and for the long-term storage of insects and other organisms at low temperature.