SBIR Phase I: Polystyrene Supports for Large Scale and Long RNA Synthesis

*9760309斯卡林格这个小型企业创新研究第一阶段项目将评估用支化链接剂衍生的无孔聚苯乙烯微珠的有效性，以产生高负载载体，并与高效的核糖核酸合成化学结合使用。这种支持将使(1)大规模的RNA合成和(2)高达120个碱基的RNA合成成为可能。该公司正在成功地开发一种RNA化学，其中每个核苷酸在90秒内偶联，产率超过99%。36个核苷酸的RNA寡核苷酸是聚苯乙烯多孔珠内外表面的功能位点。不幸的是，这些孔限制了可以合成的RNA的数量和长度。用这种化学方法在多孔载体上进行大规模合成和长序列是不可行的。提出了一种合适的无孔微珠的研制方案。寡核苷酸可以长成不受长度限制的溶液。支链连接物将使大合成的功能表面位置倍增。这些载体将用现有的RNA化学进行评估，以合成更大规模和更长的序列。项目的成功将满足研究和制药市场对大规模RNA合成和小于或相当于120个碱基的常规RNA合成的需求。这些更强大的RNA合成能力将有助于生物技术和医疗保健行业研究基于RNA的疗法。***

*** 9760309 Scaringe This Small Business Innovation Research Phase I project will evaluate the effectiveness of non-porous polystyrene beads derivatized with branched linkers to yield a high loaded support to be used in conjunction with an efficient RNA synthesis chemistry. This support will enable (1) large scale RNA syntheses and (2) syntheses of RNA up to 120 bases.The company is successfully developing an RNA chemistry in which each nucleotide couples in less than 90 seconds with yields in excess of 99%. 36-Nucleotide RNA oligonucleotides are functional sites on the external and internal surfaces of porous polystyrene beads. Unfortunately, the pores constrain the amount and length of RNA that can be synthesized. Large syntheses and long sequences are unfeasible with this chemistry on porous support. Development of a suitable non-porous bead is proposed.. Oligonucleotides would grow out into solution without limitations of length. Branched linkers would multiply functional surface sites for large syntheses. These supports will be evaluated with the existing RNA chemistry to synthesize larger scales and longer sequences. Project success will satisfy research and pharmaceutical market needs for large scale RNA syntheses and routine synthesis of RNA less than or equivalent to 120 bases long. These more powerful RNA synthesis capabilities will help the biotechnology and health care industries in investigating RNA-based therapeutics. ***