Resonance Raman Studies of Excited-State Structure and Dynamics

在化学系实验物理化学项目和分子和细胞生物学系分子生物物理项目的支持下，Mathies将继续进行生物分子的时间分辨拉曼研究。 具体地说，他提出了（I）“溶剂化电子”的结构和动力学的共振拉曼光谱研究;（II）使用飞秒拉曼增益光谱（FRGS）的视紫红质、细菌视紫红质和光敏色素中的发色团动力学的时间分辨共振拉曼光谱研究;和（III）视紫红质中蛋白质动力学的时间分辨（ns，ps）UV共振拉曼光谱研究，通过监测光解后Trp和Try的残留量来测定细菌视紫红质和光敏色素。这些研究探索了光和光生物活性材料之间相互作用的基本原理，并将有助于我们理解在有生命和无生命的生命系统中发生的基本过程。 本研究的总体目标是找到室温下凝聚相光化学系统中化学反应如何发生的一般原理。

In this project supported by the Experimental Physical Chemistry Program of the Chemistry Division and the Molecular Biophysics Program of the Division of Molecular and Cellular Biology, Mathies will continue time resolved Raman studies of biomolecules. Specifically he proposes (I) resonance Raman spectroscopic studies of the structure and dynamics of the 'solvated electron'; (II) time-resolved resonance Raman spectroscopic studies of chromophore dynamics in rhodopsin, bacteriorhodopsin and phytochrome using femtosecond Raman gain spectroscopy (FRGS); and (III) time resolved (ns, ps) UV resonance Raman spectroscopic studies of protein dynamics in rhodopsin, bacteriorhodopsin and phytochrome by monitoring the Trp and Try residues after photolysis. These investigations probe the basics of the interaction between light and photo-biologically active materials and will contribute to our understanding of the fundamental processes which take place in animate and inanimate living systems. The overall goal of this research is to find general principles about how chemical reactions occur in condensed phase photochemical systems at room temperature.