Diversity and Metabolic Capabilities of Arctic Bacterial Communities: Participation in SCICEX

本项目旨在调查北冰洋细菌群落的主要特征——丰度、群落组成和群落代谢能力的分布。研究人员计划重复在之前的sciex -95、-96和-97巡航中其他时间所做的测量，并对海冰群落进行调查。研究者将研究这些特性的时空变化模式，并试图将它们与异养生产相关的有机质来源联系起来。群落组成将采用基于分离16S rRNA基因给出的信号部分序列的方法进行分析。群落代谢能力将通过细菌在不同单一碳源电池上异养生长的能力来评估。浮游细菌丰度将由荧光显微镜测定。此外，将对水样进行分析，以确定氨基酸、碳水化合物和溶解有机碳的浓度。

This project is to investigate the distribution of key properties_abundance, community composition and community metabolic capabilities_of bacterial communities in the Arctic Ocean. The investigator plans to repeat measurements made at other times in previous SCICEX-95, -96, and -97 cruises, and to conduct investigations of sea ice communities. The investigator will examine the patterns of spatial and temporal variation of these properties and attempt to relate them to sources of organic matter associated with heterotrophic production. Community composition will be analyzed using a method based on separating partial sequences of the signal given by the 16S rRNA gene. Community metabolic capabilities will be assessed by the ability of bacteria to grow heterotrophically on a battery of different sole carbon sources. Bacterioplankton abundance will be determined by epifluorescence microscopy. In addition, water samples will be analyzed to determine the concentration of amino acids, carbohydrates, and dissolved organic carbon.