Activating Biocatalysts for Nonaqueous and Combinatorial Reactions

这一建议遵循了一个较早的观察，即某些酶催化剂在高离子、非缓冲盐（如KCl）存在下的冻干，可以将非水体系的催化速率提高3-4个数量级。这主要在枯草菌素上得到了证明。最初提议的具体目的是：阐明酶在含盐有机溶剂中活化的机制；将这一概念扩展到广泛的酶和反应。利用活性酶进行组合生物催化。在经修订和供资的提案中，删除了目标3，并增加了对这一现象的机制和范围的更详细审查。

This proposal pursues an older observation that the lyophilization of certain enzyme catalysts in the presence of highly ionic, non-buffering salts, e.g., KCl, can increase the catalytic rate in non-aqueous systems by 3-4 orders of magnitude. This has been demonstrated primarily on subtilisin. The specific aims of the original proposal are: To elucidate the mechanism(s) of enzyme activation in organic solvents with saltsTo extend the concept to a broad range of enzymes and reactions. To utilize the activated enzymes for combinatorial biocatalysis. In the revised and funded proposal, Aim 3 has been dropped and a more detailed examination of the mechanism and scope of the phenomenon has been added.