SGER: A Rapid Method for Simultaneously Quantifying and Identifying Marine Microbes

9900301，AzamA方法，如果成功的话，将同时定量和定性环境样品中存在的大多数微生物。这种方法是研究海洋微生物在海盆尺度上的分布和多样性的先决条件。该研究方法是基于这样一个事实，即一个七个碱基对的DNA标签，如果足够发散，可以区分数千种微生物。为了有用，该标签区域必须始终位于微生物的基因组内，并且它必须与可用于分离标签的保守DNA区域相关联。鉴定了RNase P基因座内的标签区域，RNase P基因座是一种普遍存在的核酶，其满足高度趋异的标准并且位于保守区域附近。幸运的是，RNase P基因座也总是以每个基因组一个拷贝的形式存在。因此，该标签可用作样品中存在的微生物物种的定量以及定性指示剂。

9900301, AzamA methodology is presented that, if successful, will simultaneously quantify and qualify the majority of microbes present in an environmental sample. Such a methodology is a prerequisite to studying the distribution and diversity ofmarine microbes on the ocean basin scale. The research method is based on the fact that a seven base pair DNA tag, if sufficiently divergent, can differentiate between thousands microbial species. To be useful this tag region must be invariably located within a microbe's genome and it must be associated with a conserved DNA region that can be used to isolate the tag A tag region within the RNase P locus, a ubiquitous nbozyme, that fulfills the criteria ofbemg highly divergent and located next to a conserved region is identified. Fortuitously, the RNase P locus is also always found as one copy per genome. Therefore, this tag can be used as a quantitative, as well as qualitative, indicator of the microbial species present in a sample.