Diversity of Marine Phage in the Pacific Ocean

太平洋海洋噬菌体的多样性

• 批准号: 9972054
• 负责人: Farooq Azam
• 金额: $ 15万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2001-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9972054&HistoricalAwards=false
• 关键词: Diversity; Marine; Phage; Pacific; Ocean

DEB 9972054 AzamThis project is designed to survey the diversity of marine phages from several sites in the Pacific Ocean. Bacteriophage are ubiquitous components of the marine environment frequently reaching concentrations of tens to hundreds of millions of individuals per ml. The decay rates of the bacteriophage are rapid, suggesting that production must be high to maintain the observed standing stocks. While numerous attempts have been made to incorporate these observations and their implications-such as mortality of marine bacteria-into the marine microbial food web, most of these studies have ignored the specificity of host/virus interactions. In fact, the diversity of marine phage is essentially unknown. Characterization of the phage isolates will be accomplished by electron microscopy, Pulse-field Gel Electrophoresis, and sequencing. These tests are specifically designed to identify the phage and classify them within known groups and to identify phage that represent novel groups. This study will be the first to systematically address the genetic diversity of this uncharacterized biota. The protocols develop and utilized here will provide the important background work for studies of phage in other environments.

Deb 9972054 Azam这个项目旨在调查太平洋几个地点的海洋噬菌体多样性。噬菌体是海洋环境中普遍存在的成分，经常达到每毫升数千万到数亿个个体的浓度。噬菌体的腐烂速度很快，这表明必须高产量才能维持观察到的常备种群。虽然许多人试图将这些观察结果及其影响--例如海洋细菌的死亡率--纳入海洋微生物食物网，但这些研究大多忽略了宿主/病毒相互作用的特殊性。事实上，海洋噬菌体的多样性基本上是未知的。噬菌体分离株的鉴定将通过电子显微镜、脉冲场凝胶电泳和测序完成。这些测试是专门设计用来识别噬菌体并将它们归类到已知的组中，以及识别代表新组的噬菌体。这项研究将是第一次系统地解决这一未知生物群的遗传多样性问题。本文所开发和使用的方法将为其他环境下噬菌体的研究提供重要的背景工作。