Assessment of the Toxicity and Endocrine Disruption Potential of Municipal, Construction and Demolition, and Industrial Solid Wastes

9906060 Bitton这是一个奖项，旨在支持含有固体废物的垃圾填埋场渗滤液的毒理学研究，这些固体废物来自含有市政和工业建筑和拆卸垃圾的垃圾填埋场。来自三个现有堆填区的渗滤液将进行研究，以确定它们的急性毒性应用程序，该程序是根据国家科学基金会补助金编号进行的研究开发的。BES 91-17267，其中开发了使用微生物分析来区分重金属和有机化学毒性的程序。在这个新项目中，将研究佛罗里达州阿拉丘亚县、柑橘县和利维县现有固体废物填埋场渗滤液的基线毒性，以确定它们的基线毒性，这些毒性可归因于它们的重金属含量，并确定它们对内分泌的潜在干扰。渗滤液还将在模拟固体废物暴露于降雨的实验室柱中产生，并使用缓冲液、去离子水和溶剂作为洗脱剂进行批量处理。这些渗滤液将通过土柱，以确定通过土壤的毒性衰减潜力。重组酵母将被用于检测混合固体废物中的环境雌激素，由笨头鱼产生的卵黄蛋白将被用作评估内分泌干扰的生物标志物。建筑和拆除活动产生的固体废物通常被放置在没有衬砌或以其他方式设计或建造以防止或限制渗滤液污染土壤和地下水的场地和垃圾填埋场。这项研究的结果可能会提供可用于确定适当的垃圾填埋场位置和工程设计的知识，以避免垃圾填埋场渗滤液对土壤、地表和地下水的污染。这项工作还有望验证可用于快速检测固体废物对环境资源的毒性、内分泌干扰和其他有害环境影响的潜在可能性的短期化验。

9906060 Bitton This is an award to support research on toxicology of leachates emerging from landfills containing solid wastes originating from landfills that contain municipal and industrial construction and demolition debris. The leachates from three existing landfills will be studied to determine their acute toxicity applying procedures developed in research conducted under NSF Grant No. BES 91-17267 in which procedures were developed to use microbial assays to distinguish between heavy metal and organic chemical toxicity. In this new project, the baseline toxicity of leachates from existing solid waste landfills in Alachua, Citrus and Levy Counties in Florida will be studied to determine their baseline toxicities, the extent to which these are attributable to their content of heavy metals and to determine their potential for endocrine disruption. Leachates will also be produced in laboratory columns simulating exposure of solid wastes to rainfall and in batch procedures using buffers, deionized water and solvents as eluants. These leachates will be passed through soil columns to determine the toxicity attenuation potential achieved by passage through soil. A recombinant yeast will be used to detect environmental estrogens in mixed solid waste and vitellogen produced by fathead minnows will be used as a biomarker for assessment of endocrine disruption. Solid wastes from construction and demolition activities are generally placed on sites and in landfills that are not lined or otherwise designed or constructed to prevent or restrain leachate from contaminating soil and groundwater. Results of this research are likely to provide knowledge that can be used to determine the appropriate placement and engineering design of landfills to avoid pollution of soil, surface and groundwater by landfill leachates. The work is also expected to validate short-term assays that can be used to rapidly detect the potential a solid waste has for toxicity, endocrine disruption and other deleterious environmental effects on environmental resources.***