FSML: A New Generation Flow Cytometer with High-Speed Sorting for Aquatic Sciences Research and Training

该项目支持购买新一代高速细胞分选的流式细胞仪，以取代Bigelow实验室j.j使用了17年的Coulter EPICS V细胞分选仪。MacIsaac流式和成像细胞仪设备。这种共享使用的仪器将提供给水生科学研究人员进行广泛的研究应用，并用于培训本科生和水生科学研究人员。除了提高可靠性之外，升级后的仪器将比现有的仪器提供几个主要优势，包括：1)提高检测亚微米颗粒的灵敏度，2)对较大颗粒（30-60 M）进行分类的能力，双激光激发能力，以及4)改进的计算机硬件和软件，使仪器设置，控制和数据分析能力更容易。获得这台仪器是毕格罗实验室科学计划的一个组成部分，以建立我们在微生物学、生理学、单细胞分析和基于物种的生态方法解决生物海洋学问题方面的能力。MacIsaac设施是一个独特的先进研究、开发和培训中心，用于研究自然水域中的颗粒的自动化方法。在过去的6年里，该设施已经为来自美国和国际上20个不同机构的25名调查人员提供了服务。它是一个创新中心，已经产生了几个新的研究和发展方向，包括最近发明的微型浮游生物（20-200克）分析的流动成像仪器。作为共享使用MacIsaac设施的一部分，新仪器将为无罪科学研究人员增加几个新的研究领域的机会。其中包括开发新的单细胞荧光方法，用于研究细菌、浮游植物和微型浮游动物的生理学，有害藻类物种的生理学，浮游生物种群的生态学研究，水生浮游生物“系统发育染色”的应用，水生颗粒的化学和光学特性，以及亚种群的分子遗传学。具有高速分选功能的新一代流式细胞仪将大大加强该技术的继续教育和培训活动。毕格罗实验室的课程和研讨会吸引了来自世界各地的研究生和博士后研究人员。现代的教育和培训手段对于保持这种领导作用是必不可少的。该仪器将被纳入j.j. MacIsaac设施，该设施一直积极服务于国际水产科学

This project supports the acquisition of a new generation flow cytometer with high-speed cell Sorting to replace the 17 year old Coulter EPICS V cell sorting instrument at Bigelow Laboratory'sJ.J. MacIsaac Facility for Flow and Imaging Cytometry. This shared-use instrument will be available to aquatic science researchers for a wide range of research applications, and for training undergraduate students, and researchers in aquatic sciences. In addition to improved reliability, the upgraded instrument will provide several major advantages over the present one including: 1) increased sensitivity for detection of submicrometer particles, 2) the ability to sort larger particles(30-60 M), dual-laser excitation capability, and 4) improved computer hardware and software for easier instrument setup, control, and data analysis capabilities. Acquisition of this instrument is an integral part of Bigelow Laboratory's scientific plan to build upon our capabilities in microbiology, phycology, single-cell analysis and species-based ecological approaches to questions in biological oceanography. The MacIsaac Facility is a unique center for advanced research, development and training in automated methods for studying particles in naturals waters. In the past 6 years the Facility has served over 25 investigators from 20 different institutions in the U.S. and internationally. It is a center for innovation, having spawned several new research and development directions, including the recent invention of an imaging-in-flow instrument for microplankton (20-200 gm) analysis. As part of the shared-use MacIsaac Facility the new instrument wil enhance several areas of new research opportunities for researchers in acquit sciences. These include the development of new single-cell fluorescence methods for studying bacteria, phytoplankton and microzooplankton physiology, the physiology of harmful algae species, ecological studies of plankton populations, the application of "phylogenetic stains" for aquatic plankton, the chemical and optical properties of aquatic particles, and the molecular genetics of subpopulations. The new generation flow cytometer with high speed sorting will significantly enhance the continued education and training activities in this technology. Bigelow Laboratory courses and workshops attract graduate students and postdoctoral researchers from around the world. A modem instrument for education and training is essential for maintaining this leadership role. The instrument wil be integrated into the J. J. MacIsaac Facility, which has actively served the international acquatic science