The Advia 120 Automated Hematology Analyzer for Complete Blood Cell Counts on Normal and Mutant Mice

9970161摘要美国杰克逊实验室（Jackson Laboratory， TJL）是公认的世界领先的哺乳动物遗传学研究机构。TJL的主要研究重点是开发用于基础生物系统研究的小鼠模型。TJL的36名研究人员中有16人在他们的研究中使用血液学分析，并且只有大约1960年的ZBI库尔特计数器可用。该奖项提供的资金将用于购买一台Advia 120自动血液分析仪。这种台式仪器将在一分钟内执行50多种不同的全血细胞参数测量。Advia 120配有兽医软件，其中包括3个预设的鼠标菌株设置。该仪器自动分离不同的血液成分——白细胞、红细胞、血小板和网织红细胞——然后用激光检测、定量和表征这些细胞。除了总计数和百分比外，Advia 120还生成每个样品的细胞成分的细胞图和直方图。长期以来，老鼠一直是理解哺乳动物遗传学和发育生物学的模式生物。通过基因靶向技术产生特定诱导突变的能力使老鼠成为一种更强大的研究动物。TJL几乎有一半的研究人员正在研究具有遗传条件的小鼠突变体，这些突变体会影响血液系统。Advia 120将允许TJL的科学家扩大他们正在进行的研究项目的范围，因为它可以快速生成50多个全血参数。Advia将用于收集新的自发突变小鼠，特定的现有已知突变小鼠的血细胞受影响，非整倍体小鼠，骨髓重建研究的数据。与TJL目前的技术相比，它提供的全面血液图谱将允许对突变小鼠进行更彻底的检查，并使研究人员能够更好地了解哺乳动物的血液系统。此外，可以探索目前不切实际的研究途径。最后，该仪器将使研究人员能够通过筛选TJL的大量血液学异常小鼠来确定血液学研究的新模型，并提供一种机制来剖析原因以及与其他非血液学遗传异常的相互作用。该仪器将由流式细胞术科学服务部保管和维护，并提供给TJL的所有研究人员。

9970161AbstractThe Jackson Laboratory (TJL) is well recognized as a world leading mammalian genetics research institution. A major focus of research at TJL is the development of mouse models for the study of basic biologic systems. Sixteen of the 36 investigators at TJL use hematological analysis in their research and only have available a Model ZBI Coulter Counter, circa 1960. Funds provided by this award will be used to purchase an Advia 120 automated hematology analyzer. This benchtop instrument will perform over 50 different whole blood cell parameter measurements in under a minute. The Advia 120 comes with veterinary software, which includes 3 preset mouse strain settings. The instrument automatically isolates the different blood components - white blood cells, red blood cells, platelets, and reticulocytes - then a laser is used to detect, quantitate, and characterize these cells. In addition to total counts and percentages, the Advia 120 produces cytograms and histograms on the cellular components of each sample.The mouse has a long tradition as a model organism for understanding mammalian genetics and developmental biology. The ability to create specific induced mutations through gene targeting technology has made the mouse an even more powerful research animal. Almost half of the researchers at TJL are studying mouse mutants with inherited conditions in which the hematologic system is affected. The Advia 120 will allow TJL scientists to expand the scope of their ongoing research projects because of the rapid generation of over 50 whole blood parameters. The Advia will be used to collect data on new spontaneous mutant mice, specific existing known mutant mice with blood cells affected, aneuploid mice, bone marrow reconstitution studies. The comprehensive blood profile it provides will allow a more thorough examination of mutant mice than is possible with the current technology at TJL and enable investigators to gain a better understanding of the mammalian hematologic system. In addition, avenues of research that are currently impractical can be explored. Finally, the instrument will enable researchers to identify new models for hematologic research by screening TJL's large holdings of mice with hematological abnormalities and provide a mechanism to dissect the causes and the interaction with other non-hematologic genetic anomalies. The instrument will be housed and maintained by the Flow Cytometry Scientific Service and made available to all researchers at TJL.