CAREER: Peptide Adsorption Dynamics on Molecularly Imprinted Surfaces: A Surface Plasmon Resonance Study

分子印迹技术为设计具有生物系统分子识别特性的材料提供了一种很有前途的技术。该过程产生的聚合材料包含目标分子的分子印记，其大小和形状尺寸与目标分子的分子印记相匹配。本研究描述了一种使用表面等离子体共振（SPR）光谱来检测分子印迹聚合物（MIP）表面上肽吸附动力学的方法。该项目有三个主要目标：i)在SPR生物传感器芯片上合成具有预定厚度的均匀超薄MIP膜；ii)实时测量目标肽在相应MIP膜上的吸附特性；iii)应用从这些测量中获得的基本信息来设计和测试用于肽分离的色谱分离材料。第一个目标将通过在目标肽存在的情况下在SPR生物传感器芯片上进行表面限制活性自由基聚合（SCLRP）来实现。SPR光谱学将用于实现第二个目标。第三个目标将通过在市售色谱支持材料上合成我们的MIP膜来实现。通过SCLRP合成MIP膜并随后使用SPR来测量这些膜的基本吸附特性代表了本研究职业规划的新特点。研究结果将广泛影响一系列MIP材料的发展，包括定制分离材料，识别和分析系统中的抗体和受体结合位点模拟物，催化酶位点模拟物以及生物传感器中的识别元件。被指派教授多门课程的小学教师经常把数学和科学列为他们最不舒服的科目。教育计划有四个主要目标：1)教导小学教师和职前教师，教数学和科学意味着与材料的互动和培养独立思考；Ii)教育教师采用探究驱动的、以活动为基础的学习方案；Iii)培训和支持教师实施这些计划；鼓励和帮助教师向其他教师传播他们的经验。

ABSTRACT CTS-9983737 S. M. Husson Clemson U.Molecular imprinting provides a promising technique for designing materials that possess the molecular recognition properties of biological systems. This process produces a polymeric material that contains a molecular imprint of a target molecule with size and shape dimensions that match those of the target molecule. This research describes a methodology that uses surface plasmon resonance (SPR) spectroscopy to examine peptide adsorption dynamics on molecularly imprinted polymer (MIP) surfaces. The project has three primary goals: i) to synthesize uniform, ultrathin MIP films with predetermined thickness on SPR biosensor chips; ii) to measure in real time the adsorption properties of target peptides on their corresponding MIP films; and iii) to apply fundamental information gained from these measurements to design and test chromatographic separation materials for peptide separations. The first goal will be achieved by carrying out surface-confined living radical polymerization (SCLRP) on the SPR biosensor chips in the presence of target peptides. SPR spectroscopy will be used to accomplish the second goal. The third goal will be met by synthesizing our MIP films on commercially available chromatography support materials. Synthesis of an MIP film by SCLRP and subsequent use of SPR to measure the fundamental adsorption properties of these films represent the novel features of this research career plan. Results of the research will broadly impact the development of a range of MIP materials, including tailor-made separation materials, antibody and receptor binding sites mimics in recognition and assay systems, enzyme site mimics for catalysis, and recognition elements in biosensors.Elementary school teachers assigned to teach multiple subjects often cite mathematics and science as their least comfortable subjects to teach. The education plan has four primary goals: i) to teach elementary school teachers and preservice teachers that teaching mathematics and science means interaction with materials and fostering independent thinking; ii) to educate teachers about programs that employ inquiry-driven, activity-based learning; iii) to train and support teachers to implement these programs; and iv) to encourage and aid teachers to disseminate their experiences to other teachers.