CAREER: Regulation of Monoclonal Antibody Production in Murine Hybridomas

本项目的目的是研究在培养的杂交瘤细胞中抗体产生的调节机制。具体的项目目标是：(1)确定在渗透胁迫下抗体产生的增加是否受到转录、翻译或翻译后过程的调节；(2)评估在渗透胁迫下应激激活和丝裂原激活的蛋白激酶级联的活性；(3)确定应激反应蛋白和伴侣蛋白在渗透胁迫反应中的作用；(4)确定细胞周期在渗透胁迫反应中的作用。(5)利用已有的抗体合成数学模型确定抗体合成的限速步骤。

The objective of this project is to investigate the mechanism by which antibody production is regulated in cultured hybridoma cells. The specific project objectives are to: (1) determine whether the increase in antibody production in response to osmotic stress is regulated by transcription, translation or post-translational processes, (2) evaluate activities of the stress-activated and mitogen-activated protein kinase cascades in response to osmotic stress, (3) establish the role of stress response proteins and chaperones in the osmotic stress response, (4) determine the role of cell cycle in osmotic stress response, and (5) use existing mathematical models of antibody synthesis to determine rate limiting steps in antibody synthesis.