GLOBEC: Applying Molecular Organic Tracers to Determine Age Structure, Nutritional Status and Possible Trophic Transfer in Euphausiids Euphausia Pacifica and Thysanoessa Spinifer

该研究项目解决了GLOBEC的两个主要目标生物，Euphausii diapacifica和Thysanoessa spinifera的种群年龄结构和营养状况。利用最新的进展，量化生化标志物的代谢（脂褐素），研究人员将确定人口年龄结构的浮游动物在加州目前的系统，特别强调人口分开的物理分区关闭中央俄勒冈州。在相同人群的子集中进行脂质分析将提供营养状况和喂养史的测量。与当前GLOBEC长期监测计划（L-TOP）相结合的采样将提供有关每个种群的物理结构的信息，以及季节性和年际时间尺度上的营养状况措施，以更好地预测其生产，招聘以及鲑鱼生长和生存的重要性。研究目标包括：1.应用生物化学方法的最新进展确定两种主要的磷虾种群的年龄结构太平洋磷虾和Thysanoessa spinifera和中尺度动力学对维持或破坏队列种群的影响季节和年际.检查物理分区的影响，在货架沃茨的营养状况，从而生殖潜力的少年和成年磷虾（和鸡蛋）使用脂质类;季节性和年际措施将被用来提供信息的潜在影响，改变营养状况对随后的招聘和丰度的变化。将联合收割机年龄结构信息与同一个体的脂肪分析相结合，以了解营养状况对野外种群年龄结构的影响。pacifica和T. spinifera在季节和年际时间尺度.探讨磷虾和其他浮游动物个体脂质作为食物分子标记的可能性及其作为向捕食者营养转移标记的可能性。

This research project addresses the population age structure and nutritional status of the two primary target organisms for GLOBEC, the euphausiids Euphausii diapacifica and Thysanoessa spinifera. Using recent advances in quantifying biochemical markers of metabolism (lipofuscins), the researchers will determine the population age structure of both zooplankton in the California current system with particular emphasis on populations separated by physical zonation off the central Oregon. Lipid analysis in subsets of the same populations will provide a measure of nutritional status and feeding history. Sampling in conjunction with the current GLOBEC long term monitoring program (L-TOP) will provide information on physical structuring of each population together with measures of nutritional status over seasonal and interannual time scales to better predict their production, recruitment and thus importance in salmonid growth and survival. The research objectives include:1. Application of recent advances in biochemical methods to determine the age structure in populations of the two major euphausiid species Euphausia pacifica and Thysanoessa spinifera and the effects of mesoscale dynamics on maintenance or disruption of cohort populations seasonally and interannually.2. Examine the effects of physical zonation in shelf waters on the nutritional status and thus reproductive potential in juvenile and adults euphausiids (and eggs) using lipid classes; seasonal and interannual measures will be used provide information on the potential effects of changing nutritional status on subsequent recruitment and variations in abundance.3. To combine age structure information with lipid analysis in sets of the same individuals to understand the impact of nutritional status on age structure in field populations of E. pacifica and T. spinifera over seasonal and interannual time scales.4. To investigate individual lipids in euphausiids and other zooplankton as molecular markers of diet and their potential as markers of trophic transfer to their predators.