Differential Expression of Somatostatin Receptors in Rainbow Trout

生长抑素(SS)是一类控制动物生长、发育和代谢的多肽激素家族。尽管这个魔力家族很重要，但人们对其多功能的基础知之甚少。具体地说，目前还不知道激素和它们的受体如何相互作用，从而在特定的靶细胞中产生特定的效果，也不知道SS家族的结构异质性如何影响这种相互作用。通过这项拟议的工作，将利用拥有多个SS的硬骨鱼(如彩虹鲑鱼)的独特情况来研究SS-受体相互作用的基本方面。彩虹鲑鱼具有多种不同配体选择性的SS受体的假说将在四个实验阶段得到验证。在第一阶段，将对编码不同SSR的多个mRNAs进行鉴定。在第二阶段，将检测表达的SS受体的结合特性。在第三阶段，将确定SS受体mRNAs的分布和丰度。在第四阶段，将确定控制SS受体mRNAs表达的因素(例如，营养状态、胰岛素)。虽然这项工作与鱼类有关，但它将为SS受体类型与特定生物学作用之间的联系提供重要的新见解，并对理解SS的多功能性质具有广泛的相关性。

Somatostatins (SS) are a diverse family of peptide hormones that controlanimal growth, development, and metabolism. Despite the importance of thishormone family, little is known about the basis of its multi-functionalnature. In particular, it is not known how the hormones and theirreceptors interact to elicit a specific effect in a particular target cellor how the structural heterogeneity of the SS family influences suchinteractions. With this proposed work, the unique situation in teleostfish such as rainbow trout, which possess multiple SSs, will be exploitedto examine fundamental aspects of SS-receptor interactions. The hypothesisthat rainbow trout possess multiple SS receptors with differential ligandselectivity that are differentially expressed will be tested in fourexperimental phases. In phase I, multiple mRNAs encoding distinct SSreceptors will be characterized. In phase 2, the binding characteristicsof expressed SS receptors will be examined. In phase 3, the distributionand abundance of SS receptor mRNAs will be determined. In phase 4, factors(e.g., nutritional state, insulin) that control the expression of SSreceptor mRNAs will be identified. While this work pertains specificallyto fish, it will provide important new insight regarding linkage between SSreceptor type to a specific biological action and have broad relevance tounderstanding the multi-functional nature of SS.