Bringing Chromatography and Spectroscopy into the Undergraduate Curriculum

生物科学（61）化学（12）在鲍登学院的科学教师努力优化本科生的学习，通过将现代分析技术纳入基于探究的实验室练习，让学生有意义的智力和创造性的投入，并通过他们对本科生主导的研究项目的监督。目前，鲍登大学主修生物学、生物化学和环境研究的本科生很少接触到色谱和光谱等基本的现代技术。化学专业的学生，虽然在这些方法的原则接地，一般不会获得与他们在复杂的分析问题的背景下的经验。本计画透过高效能液相层析法，介绍层析与光谱学的原理与应用。该项目是跨学科的性质，涉及大学生和教师从上述所有部门。该项目的具体目标是：1）通过使用二极管阵列检测的HPLC，使鲍登学院各系的本科生能够学习色谱和光谱学的原理、技术和应用，2）发展课程实验室，旨在传达这些现代技术的深刻理解，并通过使学生能够在自我创造中追求自己的假设，促进基于探究的学习，设计的实验室项目，3）促进在本科生主导的研究中使用色谱和光谱学，4）创建以HPLC技术为中心的知情课程计划，以便在科学教师中传播。色谱法和光谱学正在被纳入五个核心和高年级课程的实验室部分，以及本科生主导的研究。正在根据教育和研究文献改编实验。例如，在植物生理学课程中，科罗拉多大学开发的让学生接触光合作用和能量耗散的实验正在被修改，以使用HPLC代替TLC进行分析。总的来说，该项目正在扩大和加强鲍登本科生的背景，并为这些学生提供科学实践的第一手经验。

Biological Sciences (61) Chemistry (12)Science faculty at Bowdoin College strive to optimize undergraduate learning by incorporating modern analytical techniques into inquiry-based laboratory exercises that allow for meaningful intellectual and creative input from students, and through their oversight of undergraduate-led research projects. Currently, Bowdoin undergraduates majoring in biology, biochemistry and environmental studies receive little exposure to the essential modern techniques of chromatography and spectroscopy. Chemistry majors, although grounded in the principles of these methods, generally do not gain experience with them in the context of complex analytical problems. This project introduces undergraduates to the principles and applications of chromatography and spectroscopy via high performance liquid chromatography (HPLC). The project is interdisciplinary in nature and involves undergraduates and faculty from all of the above-mentioned departments. The specific goals of the project are to 1) enable Bowdoin undergraduates across departments to learn the principles, techniques and applications of chromatography and spectroscopy via the use of an HPLC with diode array detection, 2) develop course laboratories designed to convey deep understanding of these modern techniques and also to facilitate inquiry-based learning by enabling students to pursue hypotheses of their own creation in self-designed laboratory projects, 3) foster the use of chromatography and spectroscopy in undergraduate-led research, 4) create informed lesson plans centered on HPLC technology for dissemination among science faculty. Chromatography and spectroscopy are being incorporated into the laboratory portions of five core and upper-division courses, as well as into undergraduate-led research. Experiments are being adapted from the educational and research literature. For example, in the Plant Physiology course, experiments developed at the University of Colorado to expose students to photosynthesis and energy dissipation are being modified to use HPLC instead of TLC for the analysis. Overall, the project is broadening and enhancing the background of Bowdoin undergraduates and is providing those students with first-hand experience with the practice of science.