SBIR Phase II: In Situ Remediation of Methyl Tert-Butyl Ether (MTBE) Using Bioaugmentation

该小型企业创新研究（SBIR）第二阶段项目旨在开发和展示一种新的原位处理技术，用于破坏地下水中的甲基叔丁基醚（MTBE）。 汽油添加剂MTBE是美国第二大最普遍的地下水污染物，目前还没有经济的技术将其从供水中去除。 该技术利用了一种新的细菌的物种氢噬菌体黄（ENV 735）的甲基叔丁基醚的补救。这种细菌最近被Envirogen科学家分离出来，是仅有的两种能够在MTBE上生长的细菌菌株之一。 将进行第二阶段实验，以：（1）评估细菌在地下的移动和分布;（2）开发一种粘附缺陷菌株，以改善含水层分布;（3）优化商业规模的细菌生长、运输和注射，以供现场应用。 将进行实地示范，在现场条件下全面测试该技术。 ENV 735生物强化技术作为甲基叔丁基醚污染含水层的原位修复技术具有广泛的潜力。如果实地试验的结果是积极的，预计生物强化技术将在短期内实现商业化。

This Small Business Innovation Research (SBIR) Phase II Project is designed to develop and demonstrate a new in situ treatment technology for the destruction of methyl tert-butyl ether (MTBE) in groundwater. The gasoline additive MTBE is the second most prevalent groundwater contaminant in the United States, and there are currently no economical technologies for its removal from the water supply. This technology utilizes a novel bacterium of the species Hydrogenophaga flava (ENV735) for the remediation of MTBE. This bacterium, which was recently isolated by Envirogen scientists, is one of only two bacterial strains discovered that are capable of growth on MTBE. Phase II experiments will be conducted to: (1) assess the movement and distribution of the bacterium in the subsurface; (2) develop an adhesion-deficient strain for improved aquifer distribution; and (3) optimize commercial-scale growth, shipment, and injection of the bacterium for field application. A field demonstration will be conducted to fully test the technology under in situ conditions. The bioaugmentation with ENV735 has broad potential as an in situ remediation technology for MTBE-contaminated aquifers. If the results of the field trial are positive, commercialization of the bioaugmentation technology is anticipated in the short term.