Characterizing the Reactions of Surface-Bound Biological Molecules by Mass Spectrometry

这项研究奖给了印第安纳州大学布卢明顿的James Reilly教授，题为“通过质谱表征表面结合生物分子的反应”，由分析和表面化学计划支持。 这项研究的目标是探索蛋白质结合到表面的基本化学。 将使用质谱法探测表面相互作用。 特别是以下问题将被检查：1）与游离酶相比，结合酶的化学活性降低的原因2）影响结合蛋白质中结合位点的位置和数量的结构变化3）表面结构对酶活性的影响。 该研究在表面和界面科学以及质谱和生物化学领域具有很高的影响力。该研究具有创造性，但风险高。 它将为学生提供机会，从事涉及分析化学和蛋白质生物化学的跨学科项目。 这项研究的结果可能会影响生物技术，蛋白质诊断，蛋白质结构和建模以及免疫测定技术等领域。

This research award to Professor James Reilly of Indiana University - Bloomington, entitled "Characterizing the Reactions of Surface-bound Biological Molecules by Mass Spectrometry" is supported by the Analytical and Surface Chemistry Program. The goal of the research is to explore the fundamental chemistry of protein binding to surfaces. The surface interactions will be probed using mass spectrometry. In particular the following problems will be examined: 1) the cause of reduced chemical activity of bound enzymes compared to free enzymes 2) structural changes which affect the location and number of binding sites in bound proteins 3) the effect of the surface structure on the enzyme activity. The research has the potential for high impact in the areas of surface and interfacial science as well as for mass spectrometry and biochemistry.This research is creative but high risk. It will provide the opportunities for students to work on an interdisciplinary project involving analytical chemistry and protein biochemistry. The outcome of this research could impact the fields of biotechnology, protein diagnostics, protein structure and modeling and immunoassay techniques.