U.S.-Hungary Research on High Density Mapping of Genes Responsible for Frost Tolerance in Wheat

Dubcovsky， Davis加利福尼亚大学的Jorge Dubcovsky和Martonvasar匈牙利农业研究所的Gabor Galiba参与了这项美国和匈牙利的研究，其特点是分离和鉴定影响小麦抗冻性的基因。研究人员将把戴维斯大学开发的二倍体小麦Triticum monococum的遗传模型和工具与Martonvasar大学开发的抗冻性和春化特性鉴定程序结合起来。他们的目标是确定如何找到小麦耐霜冻的基因。利用定位克隆和差异显示相结合的方法，他们的联合研究计划包括：1)在单球菌（T. monococum）的5AmL染色体上构建影响小麦抗冻性的新基因Fr-Am2的详细染色体图谱；2)定位冷诱导单球菌抗寒性调控位点cor14b；3)利用小麦六倍体5A染色体的差异显示和缺失替换鉴定胁迫诱导基因图谱。成功鉴定的cDNA克隆将被纳入单球菌图谱。利用与抗冻基因紧密相连的分子标记，筛选单球菌细菌人工染色体文库，鉴定其完整基因及其调控元件。结果有望将控制位点与诱导基因联系起来。如果成功，新的信息可能有助于我们对抗冻性的生理生化基础的基本认识，这是一项重要的农业植物性状。这个国际植物基因组研究项目通过使美国和中欧的专家能够在共同感兴趣和能力强的领域结合互补的人才和共享研究资源，实现了推进科学知识的计划目标。

INT 0095059DubcovskyThis U.S.-Hungarian research involves Jorge Dubcovsky of the University of California, Davis, and Gabor Galiba of the Hungarian Agricultural Research Institute, Martonvasar, and features the isolation and characterization of genes affecting frost tolerance in wheat. The researchers will combine the genetic models and tools for diploid wheat, Triticum monococcum, developed at Davis with the characterization procedures for frost tolerance and vernalization developed at Martonvasar. Their goal is to determine how to cone the genes responsible for frost tolerance in wheat.Using a combination of positional cloning and differential display approaches, their joint research plan includes: 1) construction of a detailed chromosomal map for a new found gene affecting frost tolerance in wheat, Fr-Am2, on the chromosome 5AmL of T. monococcum; 2) mapping the regulatory loci of winter hardiness, cor14b, a cold-inducible expression on T. monococcum; and 3) mapping the stress induced genes, identified by differential display and deletion and substitution of chromosome 5A in hexaploid wheat. Successfully identified cDNA clones will be incorporated into T. monococcum maps.Molecular markers tightly linked to the frost tolerance genes will be used to screen the Bacterial Artificial Chromosome (BAC) library of T. monococcum to characterize the complete genes and their regulatory elements. Results are expected to relate controlling loci to inducible genes. If successful, the new information may contribute to our basic understanding of the physiological and biochemical bases for freeze tolerance, which is an agriculturally important plant trait. This international project in plant genome research fulfills the program objective of advancing scientific knowledge by enabling experts in the Untied States and Central Europe to combine complementary talents and share research resources in areas of strong mutual interest and competence.