Cellular interactions of actin regulatory proteins

与肌动蛋白细胞骨架相关的蛋白质提供信号、黏附、细胞运动和细胞内运输之间的联系。它们中的许多都具有多域结构，这使得它们可以存在于将细胞结构与细胞骨架联系起来的大型蛋白质组件中，和/或经历许多相互作用并发挥不同的作用。本研究的目的是在体内分析两种肌动蛋白结合蛋白--细丝蛋白和CAP(环化相关蛋白)的相互作用。我们将专门研究细丝蛋白S感知细胞内信号或变化的电位，并测试哪种类型的自我和非我相互作用对CAP S在体内的功能是重要的。这些研究将在Dictyostelius中进行，这使我们能够使用广泛的遗传、细胞生物学和生化技术。

Proteins associated with the actin cytoskeleton provide connections between signalling, adhesion, cell motility and intracellular transport. Many of them have a multidomain structure which allows them to be present within large protein assemblies which link cellular structures to the cytoskeleton and/or to undergo many interactions and perform diverse roles. Goal of this study is to analyse in vivo the interactions of two actin-binding proteins, filamin and CAP (cyclase associated protein). We will specifically look at filamin s potential to sense signals or changes in a cell and will test which kind of self and nonself interactions are important for CAP s in vivo function. The studies will be carried out in Dictyostelium which allows us to use a wide variety of genetic, cell biological and biochemical techniques.