SGER: High Resolution Imaging of Cancer Cells with Light Scattering Spectroscopy

建议的探索性研究的总体目标是开发一种新的高分辨率光谱成像技术，该技术利用从上皮组织近向后弹性散射的光，在细胞和亚细胞尺度上提取关于宏观组织成分以及组织结构的信息。具体目标如下：1.实验系统。开发一种实验高分辨率成像系统，用于成像混浊介质的浅层，它使用光谱和角度信息来研究组织模体、细胞和亚细胞细胞器。发展能够分离单散射光和多散射光并从组织中提取形态信息的实验技术。模特儿。开发描述单个亚细胞细胞器、细胞和细胞外组织的光散射的数学模型。识别亚细胞细胞器的光谱特征，并开发一种数学方法来提取这些细胞器的物理属性，如尺寸分布、折射率和吸收系数。实验研究。研究单个细胞、亚细胞细胞器和细胞单层的单次和多次散射的波长和角度相关性。发展基于散射光的空间分布的方法，以获得关于表层组织的组织/无序程度的信息，并使用该信息在细胞尺度上重建组织的结构图像。

0116833PerelmanThe overall objective of the proposed exploratory study is to develop a novel high resolution spectroscopic imaging technique that utilizes light, elastically scattered from epithelial tissues in near backward direction, to extract information about macroscopic tissue composition as well as tissue structure at the cellular and sub-cellular scale. The specific aims are as follows:1. Experimental system. Develop an experimental high resolution imaging system for imaging superficial layers of turbid media, which uses both spectral and angular information to study tissue phantoms, cells, and sub-cellular organelles. Develop experimental techniques capable of separating single scattered and multiply scattered light and extracting morphological information from tissue.2. Modeling. Develop mathematical models that describe light scattering from individual sub-cellular organelles, cells and extra-cellular tissues. Identify spectroscopic signatures of sub-cellular organelles, and develop a mathematical procedure for extracting physical properties of those organelles, such as size distribution, refractive indices and absorption coefficients.3. Experimental studies. Study wavelength and angular dependence of single and multiple scattering from individual cells, sub-cellular organelles, and cell monolayers. Develop methods based on spatial distribution of scattered light to obtain information about degree of organization/disorganization of superficial tissues and use this information to reconstruct structural images of tissue on a cellular scale.