US-Spain Cooperative Research: Designed Interfacial Assembly of Redox Active Enzymes and Recognition Layers for Biosensor Applications

这项为期两年的奖项旨在表彰美国和西班牙在酶固定方法方面的合作，该方法可以产生定向酶层，从而导致安培生物传感器的应用，参与者包括康奈尔大学的Hector Abruna和学生以及西班牙马德里自治大学的Encarnacion Lorenzo。酶代表了一些已知的最复杂的催化剂。将活性酶固定在固体底物上可以用来产生利用这些特征的传感器。固定方法往往会改变酶的活性，如果不是破坏酶的活性，人们对新的酶固定方法感兴趣，这种方法将允许酶层定向固定，同时保持酶的活性。美国研究人员为此次合作带来了电化学技术以及过渡金属配合物的合成和表征方面的专业知识。西班牙在各种传感器的制备和表征方面的专业知识以及它们在食品和环境样品中农药分析方面的应用也对这一点进行了补充。这项工作结合了化学和生物学的不同方面，将对环境研究和污染物监测以及生物技术应用产生兴趣和效用。

0122759AbrunaThis two-year award for US-Spain collaboration on enzyme immobilization methodologies giving rise to oriented layers of enzymes leading to amperometric biosensor applications involves Hector Abruna and students at Cornell University and Encarnacion Lorenzo at the Universidad Autonoma of Madrid in Spain. Enzymes represent some of the most sophisticated catalysts known. Immobilization of active enzymes onto solid substrates can be used to generate sensors that exploit these features. Immobilization approaches tend to alter, if not destroy, an enzyme's activity, there is interest in new enzyme immobilization methodologies that will allow for the oriented immobilization of the enzyme layer while retaining enzymatic activity.The US researchers bring to this collaboration expertise in electrochemical techniques and in the synthesis and characterization of transition metal complexes. This is complemented by Spanish expertise in the preparation and characterization of a variety of sensors and in their application to the analysis of pesticides in foods and in environmental samples. This work, coupling diverse aspects of chemistry and biology, will be of interest and utility in environmental studies and monitoring of pollutants and in biotechnological applications.