U.S.-Germany Cooperative Research: The Biochemical Function of Basonuclin in RNA Polymerase Transcription

[20202120] Tseng该奖项支持Hung Tseng和宾夕法尼亚大学的一名学生与德国海德堡德国癌症研究中心分子细胞生物学部门的Ingrid Grummt合作。该项目将重点关注碱性核素的生化功能，这是一种锌指蛋白和rRNA基因的细胞类型特异性转录调节剂。美德合作将使用生化纯化、免疫学检测以及体外和体内转录分析来研究碱性核素在聚合酶转录中的作用。所有细胞都需要rRNA转录，但在增殖和蛋白质积累过程中，当蛋白质合成速率增加时，rRNA转录的增强尤为必要。其中一个新的方面的作用是，它是细胞类型特异性，这使得它不同于所有以前描述的聚合酶I转录因子。美国方面为合作带来了有关碱性核素和试剂的最新知识。Grummt博士的实验室是世界上RNA聚合酶I研究的领先团队之一。该实验室将为聚合酶转录复合物的生化纯化和分析提供专业知识和最先进的设备。这项合作将有助于使德国和美国研究小组之间的合作关系制度化。

0202120TsengThis award supports Hung Tseng and a student from University of Pennsylvania in a collaboration with Ingrid Grummt of the Section for Molecular Cell Biology at the German Cancer Research Center in Heidelberg, Germany. The project will focus on the biochemical function of basonuclin, a zinc-finger protein and a cell-type specific transcription regulator for the rRNA genes. The US-German collaboration will use biochemical purification, immunological detection, and in vitro and in vivo transcription assays to investigate the role of basonuclin in polymerase transcription. All cell require rRNA transcription, but its enhancement is particularly necessary when the rate of protein synthesis increases in proliferation and protein accumulation. One of the novel aspects of the action of basonuclin is the it is cell-type specific, which makes it different from all previously described polymerase I transcription factors. The US side brings current knowledge about basonuclin and reagents to the collaboration. The laboratory of Dr. Grummt is one of the leading groups in the world in RNA polymerase I research. That lab will provide expertise and state-of-the-art equipment in biochemical purification and analysis of the polymerase transcription complex. This collaboration will help institutionalize the collaborative relationship between the German and US research groups.