SBIR Phase I: New Beta-Glucurondise (GUS) Substrates for Cell Regulation

这个小型企业创新研究(SBIR)第一阶段的项目是开发能够利用转化植物细胞中克隆的基因表达来控制其生长和生理的连接物的商业用途。该项目的具体目标是合成一系列新的植物调节物质的β-葡萄糖醛酸化物结合物，并测试这些结合物对表达β-葡萄糖醛酸酶(GUS)标记基因的特定组织和细胞的生长和蛋白质表达的调节能力。将在组织培养和整个植物中检测偶联物以启动子依赖的方式导致局部细胞死亡的能力。这些化合物将普遍用于植物发育和基因表达控制的研究，并有可能通过选择底物和选择报告基因和启动子元素，以可逆的方式生产具有农业重要性的植物物种。该项目的商业应用是在农业生物技术领域。

This Small Business Innovation Research (SBIR) Phase I project is to develop commercial uses for conjugates capable of exploiting cloned gene expression in transformed plant cells to control their growth and physiology. The specific goal of this project is to synthesize a series of new beta-glucuronide conjugates of plant regulatory substances and to test these conjugates for their ability to modify the growth and protein expression of specific tissues and cells expressing the beta-glucuronidase (GUS) marker gene. Conjugates will be assayed in tissue culture and in whole plants for the ability to cause localized cell death in a promoter dependent manner. Such compounds will be of general use for plant research on the control of development and gene expression and have the potential to produce agriculturally important plant species in a reversible manner, through the use of selective application of substrate and choice of reporter gene and promoter elements.The commercial application of this project is in the area of agricultural biotechnology.