MRI: Acquisition of Microscopy Instrumentation for Multi-Disciplinary Research and Research Training

摘要约翰·T·博士获得了一笔资助。Longino，Elizabeth M. Kutter和Erik V. Thuesen在万年青州立学院获得了一个可变压力扫描电子显微镜，一个倒置复合荧光显微镜和一个立体显微镜，包括电动驱动器和计算机接口，允许通过互联网进行远程操作。该仪器将用于热带生物多样性，生物膜中的微生物过程和海洋浮游动物的生态生理学研究。 约翰·朗吉诺的研究主要集中在这里和哥斯达黎加的蚂蚁上。 利用变压扫描电子显微镜和体视显微镜设备完成了对树栖蚂蚁主要属之一的哥斯达黎加火葬蚁的分类修订。 这些产品将既是传统的出版作品，也是网络上图像丰富的识别指南。 Elizabeth Kutter将使用荧光显微镜研究生物膜中细菌的噬菌体感染-非常广泛，复杂的曲折塔和流体通道社区，在很大程度上对抗生素和消毒剂具有抗药性。 Erik Thuesen正在研究凝胶状浮游动物在低氧环境中生存的能力。 他假设了一种促进扩散机制，将使用荧光显微镜检查线粒体的分布。万年青的科学教学是以研究为导向的。 许多万年青教师进行的研究和教学计划，大大依赖于显微镜，他们的能力，扩大和移动在新的方向将大大提高这些仪器。 除了P.I.的显微镜密集型研究之外，的，其他一些教师让他们的学生积极参与小规模的，当地资助的研究项目和演示，其中许多涉及复杂的显微镜。 这包括对有害藻华、环境微生物学和土壤生物学的研究。 该仪器还将用于超出这些具体的研究活动的方式，通过接触到令人兴奋的图像和概念，并提高学生的代表性不足的群体，特别是在我们的塔科马校园，这为高比例的内城学生吸引了广泛的学生。 远程控制能力将支持已经在塔科马校园启动的生物修复和微生物学方面的严肃研究项目，并将显微镜的使用从实验室扩大到课堂。 教学在万年青也是固有的和有意的跨学科，和扫描电镜和其他显微镜将作为重点全日制课程整合艺术和科学。

AbstractA grant has been awarded to Drs. John T. Longino, Elizabeth M. Kutter, and Erik V. Thuesen at The Evergreen State College to acquire a variable-pressure scanning electron microscope, an inverted compound fluorescent microscope, and a stereomicroscope, including motorized drives and computer interfaces that allow remote operation over the internet. The Instrumentation will be used In studies of tropical biodiversity, microbial processes in biofilms, and ecophysiology of marine zooplankton. John Longino's research focuses primarily on ants, here and in Costa Rica. The variable-pressure scanning electron microscope and stereomicroscope equipment will be used to finish a taxonomic revision of the Costa Rican Crematogaster, one of the major genera of arboreal ants. The products will be both traditional published work and image-rich identification guides on the web. Elizabeth Kutter will use the fluorescent microscope to study phage infections of bacteria in biofilms - very widespread, elaborate communities of convoluted towers and fluid channels that are largely resistant to antibiotics and disinfectants. Erik Thuesen is investigating the ability of gelatinous zooplankton to survive in low-oxygen environments. He hypothesizes a facilitated diffusion mechanism which will be investigated using fluorescent microscopy to examine the distribution of mitochondria. Science teaching at Evergreen is strongly research oriented. Many Evergreen faculty members carry out research and teaching programs that rely substantially on microscopy, and their ability to expand and move in new directions will be greatly enhanced by these instruments. In addition to the microscopy-intensive research of the P.I.'s, a number of other faculty involve their students actively in smaller-scale, locally-funded research projects and presentations, many of which involve sophisticated microscopy. This includes research into harmful algal blooms, environmental microbiology and soil biology. The instrumentation will also be used in ways beyond these specific research activities, attracting a broad range of students through exposure to exciting images and concepts and improving access for underrepresented groups of students, especially at our Tacoma campus, which serves a high proportion of inner city students. The remote control capabilities will support serious research projects in bioremediation and microbiology already initiated at the Tacoma campus and expand microscope use from laboratory to classroom. Teaching at Evergreen is also inherently and intentionally interdisciplinary, and the SEM and other microscopes will serve as foci for full-time programs integrating arts and sciences.