Acquisition of a 300 MHz NMR Console for Research and Training in Biochemistry and Chemistry

在化学教授Mota de Freitas博士的监督下，芝加哥洛约拉大学获得了一笔资金，用于购买一个新的300 MHz氢原子核磁共振控制台、一个变温控制模块、一个5毫米脉冲场梯度、一个间接探测探头和一个10毫米多核宽带探头。该控制台将与超导磁体一起使用，该磁体的磁场强度为7特斯拉，来自现有的、但无法操作的300窄孔核磁共振（NMR）光谱仪。核磁共振设施还拥有并维护第二台高场仪器，一台宽口径400S核磁共振光谱仪。2000年5月以前，多核300兆赫核磁共振仪主要用于研究，而400兆赫光谱仪主要用于研究生和本科生获得有机化合物的一、二维核磁共振光谱。自2000年5月起，400S核磁共振光谱仪对所有教职员工、技术人员、博士后研究人员、研究生和本科生开放。包括Mota de Freitas博士在内的五个主要研究小组在生物化学，神经化学以及生物物理，生物无机，有机金属和环境化学领域开展了一系列项目，这些项目将从专用的，升级的300 MHz核磁共振仪器中受益匪浅。核磁共振光谱学是生物化学家和化学家用来阐明分子结构的最有力的工具之一。它用于未知化合物的鉴定，分子内原子的特定排列的表征，以及溶液中分子之间相互作用的动力学方面的研究。购买的设备将用于几个项目，包括锂盐的生物无机化学研究，合成蛋白质的复杂亲和交联试剂，二羧酸连接的革兰霉素二聚体，与血红蛋白连接的光化学活性染料，利用超临界二氧化碳提取金属离子的功能化宝石双膦酸配体，钯（II）催化的不对称合成，磺酸和磺酸缓冲液的33S和17O核磁共振研究。随着300 MHz光谱仪的升级，现有的400 MHz仪器将在本科和研究生课程中得到更广泛的应用。随着本科研究计划在部门内继续扩大，另一个核磁共振光谱仪的增加将使我们的学生获得“动手”与高场光谱仪的经验。化学系举办了一个夏季REU项目，在过去的几年里，该项目广泛使用了我们的400S核磁共振光谱仪。在刚刚过去的这个夏天，所有REU的学生都接受了为期一周的核磁共振光谱仪使用概述。我们希望一台新的功能性核磁共振光谱仪能够让化学系继续整合本科生和研究生，包括女性和代表性不足的少数民族，在一个研究环境中，这将从升级的仪器中受益匪浅。

Abstract A grant has been awarded to Loyola University Chicago under the supervision of Dr. Mota de Freitas, Professor of Chemistry, for the purchase of a new 300 MHz NMR console for hydrogen atoms, a variable temperature control module, a 5 mm pulse-field gradient, an indirect-detection probe, and a 10 mm multinuclear broadband probe. The console will be used in conjunction with the superconducting magnet with a magnetic field strength of 7 Tesla from an existing, but inoperable, narrow-bore 300 Nuclear Magnetic Resonance (NMR) spectrometer. The NMR facility also owns and maintains a second high-field instrument, a wide-bore 400S NMR spectrometer. Prior to May of 2000, the multinuclear 300 MHz NMR instrument was used primarily for research whereas the 400 MHz spectrometer was used for obtaining one- and two-dimensional NMR spectra of organic compounds by graduate students as well as in undergraduate education. Since May of 2000, the 400S NMR spectrometer is accessible to all faculty, technical staff, postdoctoral researchers, graduate and advanced undergraduate students. Five principal research groups, including that of Dr. Mota de Freitas, have a series of projects in the areas of biochemistry, neurochemistry, and in biophysical, bioinorganic, organometallic and environmental chemistry that will benefit greatly from a dedicated, upgraded 300 MHz NMR instrument. NMR spectroscopy is one of the most powerful tools available to biochemists and chemists for the elucidation of the structure of molecules. It is used in the identification of unknown compounds, the characterization of specific arrangements of atoms within molecules, and in the investigation of dynamic aspects of the interactions between molecules in solution. The equipment to be purchased will be used in several projects, including studies on the bioinorganic chemistry of lithium salts, synthesis of complex affinity crosslinking reagents for proteins, dicarboxylic acid linked gramicidin dimers, photochemically active dyes linked to hemoglobin, functionalized gem-bisphosphonic acid ligands for the extraction of metal ions using supercritical carbon dioxide, asymmetric synthesis by palladium (II) catalysis, and 33S and 17O NMR studies of sulfonic acids and sulfonate buffers. With the upgraded 300 MHz spectrometer, the existing 400 MHz instrument will be used more extensively in both undergraduate and graduate classes. As the undergraduate research program within the department continues to expand, the addition of another NMR spectrometer will allow our students to gain "hands-on" experience with a high-field spectrometer. The Department of Chemistry hosts a summer REU program, which in the past few years has made extensive use of our 400S NMR spectrometer. This past summer, all REU students were exposed to a one-week overview of the use of an NMR spectrometer. It is our hope that a new functional NMR spectrometer will allow the Department of Chemistry to continue to integrate undergraduate and graduate students, including women and underrepresented minorities, in a research setting that will benefit greatly from the upgraded instrumentation.