Cultivation of novel marine bacteria of the SAR11 cluster by high throughput cultivation techniques and eluidation of their adaptations to the environment by comparative genome analysis

SAR11集群的海洋细菌可能是地球上最丰富的微生物，估计细胞数量大于2 × 1028个细胞。SAR11细菌属于（-变形菌门），不是一个同质的群体，而是由几个亚群组成的，这些亚群似乎显示出一个有趣的地理分布以及深度的空间分布。目前，SAR11分支仅有1株培养菌株，初步分类为Candidatus Pelagibacter ubique。在本应用中，将研究SAR11细菌对不同环境的适应性。利用稀释至绝灭的高通量培养方法，从百慕大大西洋时间序列研究地点（BATS）的不同深度获得细菌，培养出新的SARbe。随后的比较基因组学与已经可用的Cand基因组序列。泛层杆菌将产生首批11株基因组，以了解这些生物体对其自然栖息地的基因组适应性。

Marine bacteria of the SAR11 cluster are probably the most abundant microorganisms on earth with an estimated cell number of greater than 2 x 1028 cells. The SAR11 bacteria, belonging to the (-Proteobacteria, are not a homogenous group but consist of several subclusters that seem to show an interesting geographical distribution as well as a spatial distribution with depth. Currently, there is only one cultured strain of the SAR11 clade available, which was preliminarily classified as Candidatus Pelagibacter ubique. In this application, the adaptation of SAR11 bacteria to different environments will be studied. By using dilution-to-extinction high throughput cultivation of bacteria obtained from different depths of the well investigated Bermuda Atlantic Time-series Study site (BATS), new SARbe cultivated. Subsequent comparative genomics with the already available genome sequence of Cand. Pelagibacter ubique will result in first 11 strains will available genome t insights into the genomic adaptions of these organisms in response to their natural habitat.