Developing and Disseminating New Laboratories on Plant Molecular Genetics and Genomics

植物分子遗传学和基因组学研究仍然落后于微生物和高等动物的医学研究。因此，相对较少的实验室经验，让学生接触到基因组生物学提供的植物日益增长的见解是在较低的大学水平。这个CCLI项目正在开发一个基于实验室和互联网的课程，使学生走到现代植物研究的最前沿。 该项目主要基于冷泉港实验室（CSHL）NSF资助的主要植物研究的数据，并包括活跃研究人员的大量投入。它建立在多兰DNA学习中心16年开发和传播创新实验室课程的经验，以及作为具有强大多媒体内容和共享使用学生生成数据的互联网网站出版商的经验之上。 一套全面的实验室，基于快速和可重复的PCR化学，在先进技术教育计划的资助下开发。利用模式植物拟南芥和重要的粮食作物，实验室说明基因和基因组分析的关键概念，包括：表型和分子基因型之间的关系，植物的遗传修饰和食品中转基因的检测，以及基因作图的连锁和生物信息学方法。 学生也有独特的机会，通过协助CSHL研究人员与未知功能的拟南芥基因的细胞分析，探索功能基因组学。一个因特网“超级网站”通过在线协议、定制分析工具、共享数据库、协作公告板和聊天系统支持实验室。该建议包括一个初始开发阶段，最终是一个由来自美国六个地区的两年制和四年制大学的顾问组成的重点研讨会。在传播阶段，学院顾问将举办为期一周的培训讲习班，培训144名教员。应用生物系统公司、生物链接国家生物技术高级技术培训中心、卡罗莱纳生物供应公司和冷泉港实验室出版社致力于为该项目提供关键援助。

Plant molecular genetic and genomic research still lag behind medically-oriented research on microbes and higher animals. As a result, relatively few lab experiences that expose students to the growing insights into plants offered by genomic biology are available at the lower college level. This CCLIproject is developing a laboratory- and Internet-based curriculum to bring students to the forefront of modern plant research. The project is based largely on data emanating from major NSF-funded plant research at Cold Spring Harbor Laboratory (CSHL) and includes substantial input from active researchers. It builds on the Dolan DNA Learning Center's 16 years of experience developing and disseminating innovative laboratory curricula, and its experience as a publisher of Internet sites with strong multimedia content and shared use of student-generated data. A comprehensive set of laboratoriesis based on rapid and reproducible PCR chemistry developedunder a previous grant from the Advanced Technological Education Program. Using the model plant Arabidopsis and important food crops, the laboratories illustrate key concepts of gene and genome analysis, including: the relationship between phenotype and molecular genotype, genetic modification of plants and detection of transgenes in foods, and linkage and bioinformatic methods for gene mapping. Students also have the unique opportunity to explore functional genomics by assisting CSHL researchers with the cellular analysis of Arabidopsis genes of unknown function. An Internet "super site" supports the laboratories with online protocols, custom analysis tools, shared databases, and collaborative bulletin boards and chat systems.The proposal is comprised of an initial development phase, culminating in a focus workshop offaculty advisors drawn from two- and four-year colleges representing six regions of the United States.During the dissemination phase, faculty advisors will organize weeklong training workshops to reach 144 instructors. Applied Biosystems, Bio-Link National Center for Advanced Technological Training in Biotechnology, Carolina Biological Supply Company, and Cold Spring Harbor Laboratory Press are committed to provide key assistance to the project.