Replacement Phosphorimager for the Physical Biochemistry Instrument Facility at IUB

在Andrew Feig博士的指导下，印第安纳大学布卢明顿分校（IUB）获得了一笔赠款，用于购买磷成像仪系统，该系统将成为物理生物化学仪器设备的一部分。该设施拥有各种用于生化系统结构、光谱、热力学和动力学表征的仪器。来自至少6个系（化学、生物、心理、医学、物理和验光）的20多名教职员工使用该设施，尽管大多数用户来自化学和生物学系。通过这笔拨款获得的仪器将主要用于其磷成像仪功能，但所选择的模型也具有直接荧光成像（红色和绿色激发）和化学发光的能力。如果需要蓝色波长激发，该仪器可以在将来进行升级。该仪器还能够读取微阵列。磷成像仪将允许实验室实验使用荧光或化学发光标签代替传统的32P标签。因此，从本科实验课程中删除的优秀实验可以使用该成像系统重新合并。该仪器的微阵列读取能力也将用于课程改进，通过开发新的实验，为本科生实验室分析应激反应期间的表达谱。因此，本科生将在他们的实验课程中接触到最先进的仪器。该设备还将被50多名研究生和博士后使用。仪器的主要和次要用户积极参与IUB的几个项目（医学b， IU Stars和麦克奈尔学者），吸引对科学事业感兴趣的少数民族学生。

A grant has been awarded to Indiana University, Bloomington (IUB) under the direction of Dr. Andrew Feig for the acquisition of phosphorimager system that will become part of the Physical Biochemistry Instrumentation Facility. The facility houses a variety of instruments for structural, spectroscopic, thermodynamic and kinetic characterization of biochemical systems. More than 20 faculty members from at least 6 departments (Chemistry, Biology, Psychology, Medical Sciences, Physics and Optometry) use this facility although the majority of the users derive from the Chemistry and Biology Departments. The instrument acquired through this grant will be used primarily for its phosphorimager capabilities, but the model chosen also has the ability to do direct fluorescence imaging (red and green excitation) and chemiluminescence. The instrument can be upgraded in the future if blue wavelength excitation is needed. This instrument is also capable of reading microarrays. The phosphorimager will allow laboratory experiments that make use of fluorescent or chemiluminescent tags in lieu of traditional 32P labeling. Thus, excellent experiments that were removed from the undergraduate lab curriculum can be re-incorporated using this imaging system. The microarray reading capabilities of the instrument will also be used in curriculum enhancement through development of novel experiments for the undergraduate lab analyzing expression profiles during stress response. Thus, undergraduates will be exposed to state-of-the-art instrumentation in their laboratory course. The equipment will also be used by more than 50 graduate students and post-doctoral fellows. The major and minor users of the instrumentation are actively involved in several programs at IUB (MEDIC-B, IU Stars and McNair Scholars) that attract minority students interested in careers in science.